A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish

Soumitra Mohanty, Lakshmanan Jagannathan, Geetanjali Ganguli, Avinash Padhi, Debasish Roy, Nader Alaridah, Pratip Saha, Upendra Nongthomba, Gabriela Godaly, Ramesh Kumar Gopal, Sulagna Banerjee, Avinash Sonawane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mycobacterium tuberculosis employs various strategies to modulate host immune responses to facilitate its persistence in macrophages. The M. tuberculosis cell wall contains numerous glycoproteins with unknown roles in pathogenesis. Here, by using Concanavalin A and LC-MS analysis, we identified a novel mannosylated glycoprotein phosphoribosyltransferase, encoded by Rv3242c from M. tuberculosis cell walls. Homology modeling, bioinformatic analyses, and an assay of phosphoribosyltransferase activity in Mycobacterium smegmatis expressing recombinant Rv3242c (MsmRv3242c) confirmed the mass spectrometry data. Using Mycobacterium marinum-zebrafish and the surrogate MsmRv3242c infection models, we proved that phosphoribosyltransferase is involved in mycobacterial virulence. Histological and infection assays showed that the M. marinum mimG mutant, an Rv3242c orthologue in a pathogenic M. marinum strain, was strongly attenuated in adult zebrafish and also survived less in macrophages. In contrast, infection with wild type and the complemented &x25B5;mimG: Rv3242c M. marinum strains showed prominent pathological features, such as severe emaciation, skin lesions, hemorrhaging, and more zebrafish death. Similarly, recombinantMsmRv3242c bacteria showed increased invasion in non-phagocytic epithelial cells and longer intracellular survival in macrophages as compared with wild type and vector control M. smegmatis strains. Further mechanistic studies revealed that the Rv3242c- and mimG-mediated enhancement of intramacrophagic survival was due to inhibition of autophagy, reactive oxygen species, and reduced activities of superoxide dismutase and catalase enzymes. Infection with MsmRv3242c also activated the MAPK pathway, NF-κB, and inflammatory cytokines. In summary, we show that a novel mycobacterial mannosylated phosphoribosyltransferase acts as a virulence and immunomodulatory factor, suggesting that it may constitute a novel target for antimycobacterial drugs.

Original languageEnglish (US)
Pages (from-to)13321-13343
Number of pages23
JournalJournal of Biological Chemistry
Volume290
Issue number21
DOIs
StatePublished - May 22 2015
Externally publishedYes

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Mycobacterium marinum
Mycobacterium smegmatis
Oxidative stress
Macrophages
Autophagy
Zebrafish
Oxidative Stress
Mycobacterium tuberculosis
Assays
Glycoproteins
Infection
Cells
Cell Wall
Emaciation
Bioinformatics
Concanavalin A
Catalase
Superoxide Dismutase
Mass spectrometry
Reactive Oxygen Species

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish. / Mohanty, Soumitra; Jagannathan, Lakshmanan; Ganguli, Geetanjali; Padhi, Avinash; Roy, Debasish; Alaridah, Nader; Saha, Pratip; Nongthomba, Upendra; Godaly, Gabriela; Gopal, Ramesh Kumar; Banerjee, Sulagna; Sonawane, Avinash.

In: Journal of Biological Chemistry, Vol. 290, No. 21, 22.05.2015, p. 13321-13343.

Research output: Contribution to journalArticle

Mohanty, S, Jagannathan, L, Ganguli, G, Padhi, A, Roy, D, Alaridah, N, Saha, P, Nongthomba, U, Godaly, G, Gopal, RK, Banerjee, S & Sonawane, A 2015, 'A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish', Journal of Biological Chemistry, vol. 290, no. 21, pp. 13321-13343. https://doi.org/10.1074/jbc.M114.598482
Mohanty, Soumitra ; Jagannathan, Lakshmanan ; Ganguli, Geetanjali ; Padhi, Avinash ; Roy, Debasish ; Alaridah, Nader ; Saha, Pratip ; Nongthomba, Upendra ; Godaly, Gabriela ; Gopal, Ramesh Kumar ; Banerjee, Sulagna ; Sonawane, Avinash. / A mycobacterial phosphoribosyltransferase promotes bacillary survival by inhibiting oxidative stress and autophagy pathways in macrophages and zebrafish. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 21. pp. 13321-13343.
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AU - Padhi, Avinash

AU - Roy, Debasish

AU - Alaridah, Nader

AU - Saha, Pratip

AU - Nongthomba, Upendra

AU - Godaly, Gabriela

AU - Gopal, Ramesh Kumar

AU - Banerjee, Sulagna

AU - Sonawane, Avinash

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