A murine model for inducing and manipulating hair follicle regression (catagen): Effects of dexamethasone and cyclosporin A

Ralf Paus, Bori Handjiski, Beate M. Czarnetzki, Stefan Eichmüller

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Most cases of hair loss are based on premature induction of follicle regression (catagen). Deciphering the unknown regulation of catagen is therefore clinically important, but catagen is also an excellent model for organ involution by rapid terminal differentiation and for epithelial cell death (apoptosis). We here report an assay for the controlled pharmacologic induction and manipulation of catagen follicles. Dexamethasone-21-acetate (0.1%) was applied once daily to depilation-induced, growing follicles (anagen VI) on the backs of C57 Bl-6 mice. Characteristic catagen-associated changes in skin color were photodocumented and assessed by morphometric histology. Topical dexamethasone induced catagen-like follicles significantly earlier, more homogeneously, and also more extensively than vehicle. This process was inhibited by high intraperitoneal doses of cyclosporin A. In addition to its clinical relevance as a screening assay for catagen-blocking drugs, this simple murine model is an attractive tool for dissecting the molecular, cellular, and developmental biology of catagen.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalJournal of Investigative Dermatology
Volume103
Issue number2
DOIs
StatePublished - Aug 1994

Keywords

  • alopecia
  • apoptosis
  • glucocorticosteroids
  • hair growth

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Fingerprint Dive into the research topics of 'A murine model for inducing and manipulating hair follicle regression (catagen): Effects of dexamethasone and cyclosporin A'. Together they form a unique fingerprint.

  • Cite this