A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain

L. Mosquera, C. Forristall, Y. Zhou, M. L. King

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

mRNAs concentrated in specific regions of the oocyte have been found to encode determinants that specify cell fate. We show that an intermediate filament fraction isolated from Xenopus stage VI oocytes specifically contains, in addition to Vg1 RNA, a new localized mRNA, Xcat-2. Like Vg1, Xcat-2 is found in the vegetal cortical region, is inherited by the vegetal blastomeres during development, and is degraded very early in development. Sequence analysis suggests that Xcat-2 encodes a protein that belongs to the CCHC RNA-binding family of zinc finger proteins. Interestingly, the closest known relative to Xcat-2 in this family is nanos, an RNA localized to the posterior pole of the Drosophila oocyte whose protein product suppresses the translation of the transcription factor hunchback. The localized and maternally restricted expression of Xcat-2 RNA suggests a role for its protein in setting up regional differences in gene expression that occur early in development.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalDevelopment
Volume117
Issue number1
StatePublished - 1993

Keywords

  • Cortical cytoskeleton
  • Localized maternal RNA
  • Nanos
  • Oogenesis
  • Xenopus laevis
  • Zinc finger

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Fingerprint Dive into the research topics of 'A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain'. Together they form a unique fingerprint.

Cite this