A mouse model of intracerebral hemorrhage using autologous blood infusion

Michal A. Rynkowski, Grace H. Kim, Ricardo J Komotar, Marc L. Otten, Andrew F. Ducruet, Brad E. Zacharia, Christopher P. Kellner, David K. Hahn, Maxwell B. Merkow, Matthew C. Garrett, Robert M. Starke, Byung Moon Cho, Sergei A. Sosunov, Sander E. Connolly

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalNature Protocols
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Cerebral Hemorrhage
Blood
Stroke
Animals
Brain Edema
Basal Ganglia
Hematoma
Brain
Animal Models

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rynkowski, M. A., Kim, G. H., Komotar, R. J., Otten, M. L., Ducruet, A. F., Zacharia, B. E., ... Connolly, S. E. (2008). A mouse model of intracerebral hemorrhage using autologous blood infusion. Nature Protocols, 3(1), 122-128. https://doi.org/10.1038/nprot.2007.513

A mouse model of intracerebral hemorrhage using autologous blood infusion. / Rynkowski, Michal A.; Kim, Grace H.; Komotar, Ricardo J; Otten, Marc L.; Ducruet, Andrew F.; Zacharia, Brad E.; Kellner, Christopher P.; Hahn, David K.; Merkow, Maxwell B.; Garrett, Matthew C.; Starke, Robert M.; Cho, Byung Moon; Sosunov, Sergei A.; Connolly, Sander E.

In: Nature Protocols, Vol. 3, No. 1, 01.01.2008, p. 122-128.

Research output: Contribution to journalArticle

Rynkowski, MA, Kim, GH, Komotar, RJ, Otten, ML, Ducruet, AF, Zacharia, BE, Kellner, CP, Hahn, DK, Merkow, MB, Garrett, MC, Starke, RM, Cho, BM, Sosunov, SA & Connolly, SE 2008, 'A mouse model of intracerebral hemorrhage using autologous blood infusion', Nature Protocols, vol. 3, no. 1, pp. 122-128. https://doi.org/10.1038/nprot.2007.513
Rynkowski, Michal A. ; Kim, Grace H. ; Komotar, Ricardo J ; Otten, Marc L. ; Ducruet, Andrew F. ; Zacharia, Brad E. ; Kellner, Christopher P. ; Hahn, David K. ; Merkow, Maxwell B. ; Garrett, Matthew C. ; Starke, Robert M. ; Cho, Byung Moon ; Sosunov, Sergei A. ; Connolly, Sander E. / A mouse model of intracerebral hemorrhage using autologous blood infusion. In: Nature Protocols. 2008 ; Vol. 3, No. 1. pp. 122-128.
@article{e1397e603efb4361a2aab2dcba8d28d8,
title = "A mouse model of intracerebral hemorrhage using autologous blood infusion",
abstract = "The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.",
author = "Rynkowski, {Michal A.} and Kim, {Grace H.} and Komotar, {Ricardo J} and Otten, {Marc L.} and Ducruet, {Andrew F.} and Zacharia, {Brad E.} and Kellner, {Christopher P.} and Hahn, {David K.} and Merkow, {Maxwell B.} and Garrett, {Matthew C.} and Starke, {Robert M.} and Cho, {Byung Moon} and Sosunov, {Sergei A.} and Connolly, {Sander E.}",
year = "2008",
month = "1",
day = "1",
doi = "10.1038/nprot.2007.513",
language = "English",
volume = "3",
pages = "122--128",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - A mouse model of intracerebral hemorrhage using autologous blood infusion

AU - Rynkowski, Michal A.

AU - Kim, Grace H.

AU - Komotar, Ricardo J

AU - Otten, Marc L.

AU - Ducruet, Andrew F.

AU - Zacharia, Brad E.

AU - Kellner, Christopher P.

AU - Hahn, David K.

AU - Merkow, Maxwell B.

AU - Garrett, Matthew C.

AU - Starke, Robert M.

AU - Cho, Byung Moon

AU - Sosunov, Sergei A.

AU - Connolly, Sander E.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

AB - The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

UR - http://www.scopus.com/inward/record.url?scp=38149119767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38149119767&partnerID=8YFLogxK

U2 - 10.1038/nprot.2007.513

DO - 10.1038/nprot.2007.513

M3 - Article

C2 - 18193028

AN - SCOPUS:38149119767

VL - 3

SP - 122

EP - 128

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 1

ER -