A mouse model of intracerebral hemorrhage using autologous blood infusion

Michal A. Rynkowski, Grace H. Kim, Ricardo J. Komotar, Marc L. Otten, Andrew F. Ducruet, Brad E. Zacharia, Christopher P. Kellner, David K. Hahn, Maxwell B. Merkow, Matthew C. Garrett, Robert M. Starke, Byung Moon Cho, Sergei A. Sosunov, Sander E. Connolly

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

Original languageEnglish (US)
Pages (from-to)122-128
Number of pages7
JournalNature protocols
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Rynkowski, M. A., Kim, G. H., Komotar, R. J., Otten, M. L., Ducruet, A. F., Zacharia, B. E., Kellner, C. P., Hahn, D. K., Merkow, M. B., Garrett, M. C., Starke, R. M., Cho, B. M., Sosunov, S. A., & Connolly, S. E. (2008). A mouse model of intracerebral hemorrhage using autologous blood infusion. Nature protocols, 3(1), 122-128. https://doi.org/10.1038/nprot.2007.513