A monoclonal antibody which identifies an antigen in endothelial cell and epithelial basement membrane

Shinobu Matsuo; Neal S. Penneys; Jo David Fine; Steffen Gay; Mehrdad Nadji

doi:10.1159/000158792

A monoclonal antibody which identifies an antigen in endothelial cell and epithelial basement membrane

Shinobu Matsuo, Neal S. Penneys, Jo David Fine, Steffen Gay, Mehrdad Nadji

Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We isolated a monoclonal antibody which decorates the endothelial cells of normal and lymphatic vessels in formalin-fixed, paraffin-embedded tissue. In addition, the antibody recognizes a previously undescribed substance found in the basement membrane zone and subbasement membrane zone of a variety of epithelia. By ELISA assay, the antigen recognized by this monoclonal antibody is not laminin, type IV collagen or fibronectin. This antibody may be used as a diagnostic tool because it identifies an epitope in neoplasms differentiating towards endothelial cell such as angiosarcoma and Kaposi's sarcoma.

Original language	English (US)
Pages (from-to)	14-23
Number of pages	10
Journal	Journal of Vascular Research
Volume	27
Issue number	1
DOIs	https://doi.org/10.1159/000158792
State	Published - 1990

Keywords

basement membrane
endothelial cell
monoclonal antibody

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine

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10.1159/000158792

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abstract = "We isolated a monoclonal antibody which decorates the endothelial cells of normal and lymphatic vessels in formalin-fixed, paraffin-embedded tissue. In addition, the antibody recognizes a previously undescribed substance found in the basement membrane zone and subbasement membrane zone of a variety of epithelia. By ELISA assay, the antigen recognized by this monoclonal antibody is not laminin, type IV collagen or fibronectin. This antibody may be used as a diagnostic tool because it identifies an epitope in neoplasms differentiating towards endothelial cell such as angiosarcoma and Kaposi's sarcoma.",

keywords = "basement membrane, endothelial cell, monoclonal antibody",

author = "Shinobu Matsuo and Penneys, {Neal S.} and Fine, {Jo David} and Steffen Gay and Mehrdad Nadji",

year = "1990",

doi = "10.1159/000158792",

language = "English (US)",

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pages = "14--23",

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AU - Matsuo, Shinobu

AU - Penneys, Neal S.

AU - Fine, Jo David

AU - Gay, Steffen

AU - Nadji, Mehrdad

PY - 1990

Y1 - 1990

N2 - We isolated a monoclonal antibody which decorates the endothelial cells of normal and lymphatic vessels in formalin-fixed, paraffin-embedded tissue. In addition, the antibody recognizes a previously undescribed substance found in the basement membrane zone and subbasement membrane zone of a variety of epithelia. By ELISA assay, the antigen recognized by this monoclonal antibody is not laminin, type IV collagen or fibronectin. This antibody may be used as a diagnostic tool because it identifies an epitope in neoplasms differentiating towards endothelial cell such as angiosarcoma and Kaposi's sarcoma.

AB - We isolated a monoclonal antibody which decorates the endothelial cells of normal and lymphatic vessels in formalin-fixed, paraffin-embedded tissue. In addition, the antibody recognizes a previously undescribed substance found in the basement membrane zone and subbasement membrane zone of a variety of epithelia. By ELISA assay, the antigen recognized by this monoclonal antibody is not laminin, type IV collagen or fibronectin. This antibody may be used as a diagnostic tool because it identifies an epitope in neoplasms differentiating towards endothelial cell such as angiosarcoma and Kaposi's sarcoma.

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KW - endothelial cell

KW - monoclonal antibody

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A monoclonal antibody which identifies an antigen in endothelial cell and epithelial basement membrane

Abstract

Keywords

ASJC Scopus subject areas

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