A modified procedure for the determination of 5-hydroxyindoleacetic acid in the urine and cerebrospinal fluid of patients with hepatic cirrhosis

Roy S. Dombro, Duane G. Hutson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A method has been developed for the separation of 5-hydroxyindoleacetic acid from substances in urine and cerebrospinal fluid which interfere with its fluorometric determination. This involves the use of anion exchange column chromatography with elution of the 5-hydroxyindoleacetic acid by 3 mol/1 formic acid in 60% methanol. The indole acid is then assayed fluorometrically after reaction with o-phtalaldehyde. Urinary 5-hydroxyindoleacetic acid values are reported for patients with stable cirrhosis and hepatic encephalopathy and compared with those found in control subjects without liver disease.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalClinica Chimica Acta
Volume100
Issue number3
DOIs
StatePublished - Jan 31 1980

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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