Abstract
Oxidation of araldite-embedded liver sections by 1% w/v aqueous H5IO6 for 15 min and a 5-min reaction of carbonyls with 1% w/v thiocarbohydrazide in 10% v/v acetic acid was employed for subsequent staining of glycogen with silver-proteinate (S-P). The network of branching intracellular glycogen aggregates was revealed by 15-min staining with S-P, whereas 24 hr incubation in S-P was necessary to enhance the contrast of glycogen inclusions. We conclude that the proposed modification of glycogen staining readily affords the means for its localization at a desired level of contrast and resolution.
| Original language | English |
|---|---|
| Pages (from-to) | 393-399 |
| Number of pages | 7 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 35 |
| Issue number | 3 |
| State | Published - Jan 1 1987 |
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ASJC Scopus subject areas
- Cell Biology
- Anatomy
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A modified periodic acid-thiocarbohydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. / Lo, H. K.; Malinin, Theodore; Malinin, G. I.
In: Journal of Histochemistry and Cytochemistry, Vol. 35, No. 3, 01.01.1987, p. 393-399.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A modified periodic acid-thiocarbohydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy
AU - Lo, H. K.
AU - Malinin, Theodore
AU - Malinin, G. I.
PY - 1987/1/1
Y1 - 1987/1/1
N2 - Oxidation of araldite-embedded liver sections by 1% w/v aqueous H5IO6 for 15 min and a 5-min reaction of carbonyls with 1% w/v thiocarbohydrazide in 10% v/v acetic acid was employed for subsequent staining of glycogen with silver-proteinate (S-P). The network of branching intracellular glycogen aggregates was revealed by 15-min staining with S-P, whereas 24 hr incubation in S-P was necessary to enhance the contrast of glycogen inclusions. We conclude that the proposed modification of glycogen staining readily affords the means for its localization at a desired level of contrast and resolution.
AB - Oxidation of araldite-embedded liver sections by 1% w/v aqueous H5IO6 for 15 min and a 5-min reaction of carbonyls with 1% w/v thiocarbohydrazide in 10% v/v acetic acid was employed for subsequent staining of glycogen with silver-proteinate (S-P). The network of branching intracellular glycogen aggregates was revealed by 15-min staining with S-P, whereas 24 hr incubation in S-P was necessary to enhance the contrast of glycogen inclusions. We conclude that the proposed modification of glycogen staining readily affords the means for its localization at a desired level of contrast and resolution.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023137354&partnerID=8YFLogxK
M3 - Article
C2 - 2434558
AN - SCOPUS:0023137354
VL - 35
SP - 393
EP - 399
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 3
ER -