Abstract
The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 215-223 |
| Number of pages | 9 |
| Journal | Journal of Biomolecular Structure and Dynamics |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Structural Biology
Cite this
A model for the solution structure of a branched, three-strand dna complex. / Leontis, Neocles B.; Hills, Michael T.; Piotto, Martial; Malhotra, Arun; Nussbaum, Jean; Orenstein, David G.
In: Journal of Biomolecular Structure and Dynamics, Vol. 11, No. 2, 1993, p. 215-223.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A model for the solution structure of a branched, three-strand dna complex
AU - Leontis, Neocles B.
AU - Hills, Michael T.
AU - Piotto, Martial
AU - Malhotra, Arun
AU - Nussbaum, Jean
AU - Orenstein, David G.
PY - 1993
Y1 - 1993
N2 - The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.
AB - The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.
UR - http://www.scopus.com/inward/record.url?scp=0027441897&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027441897&partnerID=8YFLogxK
U2 - 10.1080/07391102.1993.10508722
DO - 10.1080/07391102.1993.10508722
M3 - Article
C2 - 8286052
AN - SCOPUS:0027441897
VL - 11
SP - 215
EP - 223
JO - Journal of Biomolecular Structure and Dynamics
JF - Journal of Biomolecular Structure and Dynamics
SN - 0739-1102
IS - 2
ER -