A model for the solution structure of a branched, three-strand dna complex

Neocles B. Leontis, Michael T. Hills, Martial Piotto, Arun Malhotra, Jean Nussbaum, David G. Orenstein

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalJournal of Biomolecular Structure and Dynamics
Volume11
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

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Thymidine
Guanosine
DNA
Oligonucleotides
Nucleic Acids
Magnetic Resonance Spectroscopy
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

A model for the solution structure of a branched, three-strand dna complex. / Leontis, Neocles B.; Hills, Michael T.; Piotto, Martial; Malhotra, Arun; Nussbaum, Jean; Orenstein, David G.

In: Journal of Biomolecular Structure and Dynamics, Vol. 11, No. 2, 1993, p. 215-223.

Research output: Contribution to journalArticle

Leontis, Neocles B. ; Hills, Michael T. ; Piotto, Martial ; Malhotra, Arun ; Nussbaum, Jean ; Orenstein, David G. / A model for the solution structure of a branched, three-strand dna complex. In: Journal of Biomolecular Structure and Dynamics. 1993 ; Vol. 11, No. 2. pp. 215-223.
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