A model for the solution structure of a branched, three-strand dna complex

Neocles B. Leontis; Michael T. Hills; Martial Piotto; Arun Malhotra; Jean Nussbaum; David G. Orenstein

doi:10.1080/07391102.1993.10508722

A model for the solution structure of a branched, three-strand dna complex

Neocles B. Leontis, Michael T. Hills, Martial Piotto, Arun Malhotra, Jean Nussbaum, David G. Orenstein

Research output: Contribution to journal › Article

30 Scopus citations

Abstract

The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.

Original language	English (US)
Pages (from-to)	215-223
Number of pages	9
Journal	Journal of Biomolecular Structure and Dynamics
Volume	11
Issue number	2
DOIs	https://doi.org/10.1080/07391102.1993.10508722
State	Published - Oct 1993
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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Leontis, N. B., Hills, M. T., Piotto, M., Malhotra, A., Nussbaum, J., & Orenstein, D. G. (1993). A model for the solution structure of a branched, three-strand dna complex. Journal of Biomolecular Structure and Dynamics, 11(2), 215-223. https://doi.org/10.1080/07391102.1993.10508722

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abstract = "The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.",

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AU - Leontis, Neocles B.

AU - Hills, Michael T.

AU - Piotto, Martial

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AU - Nussbaum, Jean

AU - Orenstein, David G.

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N2 - The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.

AB - The solution structure of a DNA three-way junction (TWJ) containing two unpaired thymidines was elucidated using two-and three-dimensional !H nuclear magnetic resonance (NMR) spectroscopy. TWJs with unpaired nucleotides are ubiquitous structural motifs of complex single-stranded nucleic acids. In the presence of Mg2+. the TWJ complex adopts a unique conformation in which the bases of one of the oligonucleotides (“strand 1”) are continuously stacked across the junction. Guanosine 8 of strand 3(S3-G8), which pairs with S1-C5. stacks on S2-G5, which is paired to S1-C6. The unpaired thymidine bases (S3-T6 and S3-T7) are exposed to the solvent, whereas the sugar of S3-G8 is largely buried. S3-T6 also interacts with the sugar residue of S3-G11. All three stems conform to B-type DNA.

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