A minimally invasive technique for intrathymic cell transplantation in the dog

Robert D. Schachner, Tie Qian, Sheryl Strasser, Mathias D. Brendel, Rodolfo Alejandro, Daniel H. Mintz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We show an approach to the dog thymus using fiber optics for injection of bone marrow (BM) cells as the tolerogen. Bone marrow was retrieved from the donor beagles and purified using an automated Ficoll-Paque gradient technique. The purified cellular suspension was injected into the thymus through a small intercostal incision with the use of an injection needle port guided by the use of a rigid fiberoptic scope. To demonstrate engraftment, supravital staining with Fluorescein Diacetate of the BM cells was performed prior to inoculation. Immunofluorescence of cryostat sections obtained at necropsy confirmed the presence of viable BM cells up to several days after transplantation. Results of this study show that the thoracoscopic approach to the thymus can be safely and effectively used for IT inoculation studies in dogs.

Original languageEnglish
Pages (from-to)349-350
Number of pages2
JournalCell Transplantation
Volume3
Issue number4
StatePublished - Jul 1 1994

Fingerprint

Cell Transplantation
Thymus
Bone Marrow Cells
Thymus Gland
Bone
Tissue Donors
Dogs
Fiber optics
Ficoll
Thoracoscopy
Injections
Immunosuppression
Transplants
Needles
Cryostats
Fluorescent Antibody Technique
Suspensions
Transplantation
Bone Marrow
Staining and Labeling

Keywords

  • Dog model
  • Thoracoscopy
  • Thymus inoculation

ASJC Scopus subject areas

  • Transplantation
  • Cell Biology

Cite this

Schachner, R. D., Qian, T., Strasser, S., Brendel, M. D., Alejandro, R., & Mintz, D. H. (1994). A minimally invasive technique for intrathymic cell transplantation in the dog. Cell Transplantation, 3(4), 349-350.

A minimally invasive technique for intrathymic cell transplantation in the dog. / Schachner, Robert D.; Qian, Tie; Strasser, Sheryl; Brendel, Mathias D.; Alejandro, Rodolfo; Mintz, Daniel H.

In: Cell Transplantation, Vol. 3, No. 4, 01.07.1994, p. 349-350.

Research output: Contribution to journalArticle

Schachner, RD, Qian, T, Strasser, S, Brendel, MD, Alejandro, R & Mintz, DH 1994, 'A minimally invasive technique for intrathymic cell transplantation in the dog', Cell Transplantation, vol. 3, no. 4, pp. 349-350.
Schachner RD, Qian T, Strasser S, Brendel MD, Alejandro R, Mintz DH. A minimally invasive technique for intrathymic cell transplantation in the dog. Cell Transplantation. 1994 Jul 1;3(4):349-350.
Schachner, Robert D. ; Qian, Tie ; Strasser, Sheryl ; Brendel, Mathias D. ; Alejandro, Rodolfo ; Mintz, Daniel H. / A minimally invasive technique for intrathymic cell transplantation in the dog. In: Cell Transplantation. 1994 ; Vol. 3, No. 4. pp. 349-350.
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