A micro double capillary method for rheologic measurements of lower airway secretions

C. S. Kim, B. B. Berkley, W. M. Abraham, Adam Wanner

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We modified the double capillary method of Philipoff et al. to allow rheologic measurements of lower respiratory secretions. The system consists of a precision bore stainless steel capillary (0.58 mm i.d., 5 mm length), a glass capillary (0.92 mm i.d., 15 mm length) and a sample holding tube (1.5 mm i.d., 15 mm length). This series of capillaries is connected to a long polyethylene catheter which permits advancing the system through the inner channel of a fiberoptic bronchoscope, and the placing of the sample holding tube into the lower airways for the aspiration of secretions under direct vision. The required sample volume is 20 μl. The capillary is then removed from the fiberoptic bronchoscope and placed on a microscope stage; the movement of the meniscus of the sample under an applied vacuum as well as the elastic recoil of the sample after release of the vacuum are measured at body temperature. From this procedure, apparent viscosity (ηa) and shear elastic modulus (G) are calculated. The accuracy of the method was verified in vitro by using standard viscosity oils and a polymer solution of known elasticity. In five normal conscious sheep, ηa ranged between 200-3000 poises (shear rate: 0.06-1.0 s-1) and G between 17-83 dyn x cm-2. The micro double capillary method was found adequate for direct rheologic measurements of lower airway secretions.

Original languageEnglish
Pages (from-to)915-927
Number of pages13
JournalClinical Respiratory Physiology
Volume18
Issue number6
StatePublished - Dec 1 1982

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Bronchoscopes
Vacuum
Viscosity
Elastic Modulus
Stainless Steel
Elasticity
Polyethylene
Body Temperature
Glass
Sheep
Polymers
Oils
Catheters
Aspirations (Psychology)
In Vitro Techniques
Meniscus

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

A micro double capillary method for rheologic measurements of lower airway secretions. / Kim, C. S.; Berkley, B. B.; Abraham, W. M.; Wanner, Adam.

In: Clinical Respiratory Physiology, Vol. 18, No. 6, 01.12.1982, p. 915-927.

Research output: Contribution to journalArticle

Kim, C. S. ; Berkley, B. B. ; Abraham, W. M. ; Wanner, Adam. / A micro double capillary method for rheologic measurements of lower airway secretions. In: Clinical Respiratory Physiology. 1982 ; Vol. 18, No. 6. pp. 915-927.
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