A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys

Y. Yasutomi, S. N. McAdam, J. E. Boyson, M. S. Piekarczyk, David Watkins, N. L. Letvin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In light of the importance of virus-specific CTL in the control of the spread of the AIDS virus, it will be important to assess the generation of these effector cell responses in trials of novel vaccine strategies for the prevention of AIDS virus infections. To facilitate such studies in the simian immunodeficiency virus (SIV)/macaque model for AIDS, we have defined a rhesus monkey SIV(mac) CTL epitope carboxy terminus to both the CD4-binding and V4 regions of the envelope glycoprotein. We also used one-dimensional isoelectric focusing to characterize the MHC class I molecule of the rhesus monkey that binds this 9-amino-acid SIV(mac) envelope fragment. Cloning and sequencing of the cDNA encoding this rhesus monkey MHC class I molecule demonstrated that it is a newly described HLA-B homologue, Mamu-B*01. The definition of this viral CTL epitope and its restricting MHC class I molecule will facilitate the use of the SIV(mac)/rhesus monkey model for studies of envelope-based vaccine strategies for the prevention of ADS.

Original languageEnglish
Pages (from-to)2516-2522
Number of pages7
JournalJournal of Immunology
Volume154
Issue number5
StatePublished - Jan 1 1995
Externally publishedYes

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Simian Immunodeficiency Virus
Macaca mulatta
Epitopes
Alleles
Vaccines
HIV
HLA-B Antigens
Isoelectric Focusing
Macaca
Virus Diseases
Organism Cloning
Glycoproteins
Acquired Immunodeficiency Syndrome
Complementary DNA
Viruses
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Yasutomi, Y., McAdam, S. N., Boyson, J. E., Piekarczyk, M. S., Watkins, D., & Letvin, N. L. (1995). A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys. Journal of Immunology, 154(5), 2516-2522.

A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys. / Yasutomi, Y.; McAdam, S. N.; Boyson, J. E.; Piekarczyk, M. S.; Watkins, David; Letvin, N. L.

In: Journal of Immunology, Vol. 154, No. 5, 01.01.1995, p. 2516-2522.

Research output: Contribution to journalArticle

Yasutomi, Y, McAdam, SN, Boyson, JE, Piekarczyk, MS, Watkins, D & Letvin, NL 1995, 'A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys', Journal of Immunology, vol. 154, no. 5, pp. 2516-2522.
Yasutomi, Y. ; McAdam, S. N. ; Boyson, J. E. ; Piekarczyk, M. S. ; Watkins, David ; Letvin, N. L. / A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys. In: Journal of Immunology. 1995 ; Vol. 154, No. 5. pp. 2516-2522.
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