A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys

Y. Yasutomi, S. N. McAdam, J. E. Boyson, M. S. Piekarczyk, D. I. Watkins, N. L. Letvin

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

In light of the importance of virus-specific CTL in the control of the spread of the AIDS virus, it will be important to assess the generation of these effector cell responses in trials of novel vaccine strategies for the prevention of AIDS virus infections. To facilitate such studies in the simian immunodeficiency virus (SIV)/macaque model for AIDS, we have defined a rhesus monkey SIV(mac) CTL epitope carboxy terminus to both the CD4-binding and V4 regions of the envelope glycoprotein. We also used one-dimensional isoelectric focusing to characterize the MHC class I molecule of the rhesus monkey that binds this 9-amino-acid SIV(mac) envelope fragment. Cloning and sequencing of the cDNA encoding this rhesus monkey MHC class I molecule demonstrated that it is a newly described HLA-B homologue, Mamu-B*01. The definition of this viral CTL epitope and its restricting MHC class I molecule will facilitate the use of the SIV(mac)/rhesus monkey model for studies of envelope-based vaccine strategies for the prevention of ADS.

Original languageEnglish (US)
Pages (from-to)2516-2522
Number of pages7
JournalJournal of Immunology
Volume154
Issue number5
StatePublished - 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys'. Together they form a unique fingerprint.

  • Cite this

    Yasutomi, Y., McAdam, S. N., Boyson, J. E., Piekarczyk, M. S., Watkins, D. I., & Letvin, N. L. (1995). A MHC class I B locus allele-restricted simian immunodeficiency virus envelope CTL epitope in rhesus monkeys. Journal of Immunology, 154(5), 2516-2522.