A method of determining DNA and chondrocyte content of articular cartilage

Herman S. Cheung, Lawrence M. Ryan

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A novel and precise method was devised to study the DNA and chondrocyte content of articular cartilage. It involved the sequential digestion of cartilage matrix with hyaluronidase, trypsin, and collagenase to release the chondrocytes. A direct cell count and DNA assays were then performed on the cells. The concentration of cells was the quotient of the total number of cells and the weight of cartilage used. The DNA content of cartilage is identical to the amount of DNA in the chondrocytes. Our data also confirmed the earlier findings that cell density and DNA content of articular cartilage decreased gradually to a relatively constant level as animals matured to adulthood.

Original languageEnglish (US)
Pages (from-to)93-97
Number of pages5
JournalAnalytical Biochemistry
Volume116
Issue number1
DOIs
StatePublished - Sep 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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