A method for the mass isolation of islets from the adult pig pancreas

Camillo Ricordi, E. H. Finke, P. E. Lacy

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in vitro.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalDiabetes
Volume35
Issue number6
StatePublished - Jan 1 1986
Externally publishedYes

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Pancreas
Swine
Ficoll
Digestion
Glucose

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

A method for the mass isolation of islets from the adult pig pancreas. / Ricordi, Camillo; Finke, E. H.; Lacy, P. E.

In: Diabetes, Vol. 35, No. 6, 01.01.1986, p. 649-653.

Research output: Contribution to journalArticle

Ricordi, C, Finke, EH & Lacy, PE 1986, 'A method for the mass isolation of islets from the adult pig pancreas', Diabetes, vol. 35, no. 6, pp. 649-653.
Ricordi, Camillo ; Finke, E. H. ; Lacy, P. E. / A method for the mass isolation of islets from the adult pig pancreas. In: Diabetes. 1986 ; Vol. 35, No. 6. pp. 649-653.
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