A method for the mass isolation of islets from the adult pig pancreas

C. Ricordi, E. H. Finke, P. E. Lacy

Research output: Contribution to journalArticle

155 Scopus citations

Abstract

A method for mass isolation of islets from the pig pancreas is described. The procedure involves the use of an enzymatic and mechanical pancreatic digestion procedure followed by filtration and separation of the islets on Ficoll gradients. A remarkably high yield of purified islets has been obtained from the pig pancreas with this procedure. The islets are morphologically intact and respond to acute stimulation with glucose in vitro.

Original languageEnglish (US)
Pages (from-to)649-653
Number of pages5
JournalDiabetes
Volume35
Issue number6
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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