A method for freezing rat kidney in situ

Thomas E. Wheeler; E. Dewey Thomas; Theodore Malinin

doi:10.1097/00007890-196407000-00011

A method for freezing rat kidney in situ

Thomas E. Wheeler, E. Dewey Thomas, Theodore Malinin

Orthopaedics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A surgical approach has been described which permits mobilization and perfusion of the rat kidney as a preliminary to in situ freezing of this organ. The freezing apparatus employed was fabricated in the laboratory. The freezing chamber consisted of a rubber condom placed around the kidney and attached to a large glass tube. The slope of the freezing curve is dictated by a controller-recorder apparatus. The freezing apparatus and operative technique was evaluated as to its practicability by freezing rat kidneys perfused with 13% dimethylsulfoxide. There was no evidence of post-freezing renal function in these experiments. Histological studies indicated that portions of the kidneys perfused with dimethylsulfoxide survived.

Original language	English (US)
Pages (from-to)	534-540
Number of pages	7
Journal	Transplantation
Volume	2
Issue number	4
DOIs	https://doi.org/10.1097/00007890-196407000-00011
State	Published - Jul 1964

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Transplantation

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AB - A surgical approach has been described which permits mobilization and perfusion of the rat kidney as a preliminary to in situ freezing of this organ. The freezing apparatus employed was fabricated in the laboratory. The freezing chamber consisted of a rubber condom placed around the kidney and attached to a large glass tube. The slope of the freezing curve is dictated by a controller-recorder apparatus. The freezing apparatus and operative technique was evaluated as to its practicability by freezing rat kidneys perfused with 13% dimethylsulfoxide. There was no evidence of post-freezing renal function in these experiments. Histological studies indicated that portions of the kidneys perfused with dimethylsulfoxide survived.

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A method for freezing rat kidney in situ

Abstract

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