Abstract
A surgical approach has been described which permits mobilization and perfusion of the rat kidney as a preliminary to in situ freezing of this organ. The freezing apparatus employed was fabricated in the laboratory. The freezing chamber consisted of a rubber condom placed around the kidney and attached to a large glass tube. The slope of the freezing curve is dictated by a controller-recorder apparatus. The freezing apparatus and operative technique was evaluated as to its practicability by freezing rat kidneys perfused with 13% dimethylsulfoxide. There was no evidence of post-freezing renal function in these experiments. Histological studies indicated that portions of the kidneys perfused with dimethylsulfoxide survived.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 534-540 |
| Number of pages | 7 |
| Journal | Transplantation |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 1964 |
ASJC Scopus subject areas
- Transplantation
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Wheeler, T. E., Dewey Thomas, E., & Malinin, T. (1964). A method for freezing rat kidney in situ. Transplantation, 2(4), 534-540. https://doi.org/10.1097/00007890-196407000-00011