A method for culturing embryonic C. elegans cells

Rachele Sangaletti, Laura Bianchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

C. elegans is a powerful model system, in which genetic and molecular techniques are easily applicable. Until recently though, techniques that require direct access to cells and isolation of specific cell types, could not be applied in C. elegans. This limitation was due to the fact that tissues are confined within a pressurized cuticle which is not easily digested by treatment with enzymes and/or detergents. Based on early pioneer work by Laird Bloom, Christensen and colleagues 1 developed a robust method for culturing C. elegans embryonic cells in large scale. Eggs are isolated from gravid adults by treatment with bleach/NaOH and subsequently treated with chitinase to remove the eggshells. Embryonic cells are then dissociated by manual pipetting and plated onto substrate-covered glass in serum-enriched media. Within 24 hr of isolation cells begin to differentiate by changing morphology and by expressing cell specific markers. C. elegans cells cultured using this method survive for up 2 weeks in vitro and have been used for electrophysiological, immunochemical, and imaging analyses as well as they have been sorted and used for microarray profiling.

Original languageEnglish
Article numbere50649
JournalJournal of Visualized Experiments
Issue number79
DOIs
StatePublished - Sep 21 2013

Fingerprint

Chitinases
Detergents
Microarrays
Enzymes
Tissue
Imaging techniques
Glass
Cell Separation
Substrates
Egg Shell
Genetic Techniques
Eggs
Cultured Cells
Serum

Keywords

  • Biological phenomena
  • C. elegans
  • Cell culture
  • Cell physiological phenomena
  • Developmental biology
  • Embryonic cells
  • Eukaryota
  • Issue 79

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

A method for culturing embryonic C. elegans cells. / Sangaletti, Rachele; Bianchi, Laura.

In: Journal of Visualized Experiments, No. 79, e50649, 21.09.2013.

Research output: Contribution to journalArticle

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