A mechanism to signal receptor-substrate interactions with luminescent quantum dots

Ibrahim Yildiz, Massimiliano Tomasulo, Francisco Raymo

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.

Original languageEnglish
Pages (from-to)11457-11460
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number31
DOIs
StatePublished - Aug 1 2006

Fingerprint

Quantum Dots
Luminescence
Electrons
Luminescent Measurements
Semiconductors
Static Electricity
Nanoparticles
Adsorption
Ligands
Proteins

Keywords

  • Electron transfer
  • Luminescent chemosensors
  • Nanoparticles
  • Protein-ligand interactions

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A mechanism to signal receptor-substrate interactions with luminescent quantum dots. / Yildiz, Ibrahim; Tomasulo, Massimiliano; Raymo, Francisco.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 31, 01.08.2006, p. 11457-11460.

Research output: Contribution to journalArticle

@article{dd95a939ed5d47a79ee69f87702a1c08,
title = "A mechanism to signal receptor-substrate interactions with luminescent quantum dots",
abstract = "Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.",
keywords = "Electron transfer, Luminescent chemosensors, Nanoparticles, Protein-ligand interactions",
author = "Ibrahim Yildiz and Massimiliano Tomasulo and Francisco Raymo",
year = "2006",
month = "8",
day = "1",
doi = "10.1073/pnas.0602384103",
language = "English",
volume = "103",
pages = "11457--11460",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "31",

}

TY - JOUR

T1 - A mechanism to signal receptor-substrate interactions with luminescent quantum dots

AU - Yildiz, Ibrahim

AU - Tomasulo, Massimiliano

AU - Raymo, Francisco

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.

AB - Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.

KW - Electron transfer

KW - Luminescent chemosensors

KW - Nanoparticles

KW - Protein-ligand interactions

UR - http://www.scopus.com/inward/record.url?scp=33746796829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746796829&partnerID=8YFLogxK

U2 - 10.1073/pnas.0602384103

DO - 10.1073/pnas.0602384103

M3 - Article

C2 - 16861301

AN - SCOPUS:33746796829

VL - 103

SP - 11457

EP - 11460

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 31

ER -