A maxi Cl- channel in cultured pavement cells from the gills of the freshwater rainbow trout Oncorhynchus mykiss

M. J. O'Donnell, S. P. Kelly, C. A. Nurse, C. M. Wood

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Primary cultures of pavement cells from the gills of a freshwater fish, the rainbow trout Oncorhynchus mykiss, have been studied for the first time using the patch-clamp technique. Gigaohm seals were obtained in approximately 95 % of cells studied, and channel activity was evident in a high proportion (>90 %). A large-conductance Cl- channel was evident in 6 % of cell-attached and in 31 % of inside-out patches. Single-channel conductance in inside-out patches was 372 pS, and current/voltage relationships were linear over the range -60 to +60 mV. The channel was activated by patch excision, and activation was often associated with polarization of the patch. The mean number of channels per patch was 1.9, and there were several subconductance states. The relationship between channel activity (NP0) and voltage was in the form of an inverted U, and channel activity was highest between 0 and +20mV. Large-conductance Cl- channels showed a progressive time-dependent reduction in current in response to sustained polarization to voltages outside the range -20 mV to +20 mV. Permeability ratios (P) of Cl- to other anions were PHCO3/PCl=0.81, PSO4/PCl=0.31 and Pisethionate/PCl=0.53. The channel was blocked by Zn2+, SITS, DIDS and diphenylamine carboxylate. This is the first description of a large-conductance Cl- channel in gill cells from freshwater or marine species. Possible functions of the channel are discussed.

Original languageEnglish
Pages (from-to)1783-1794
Number of pages12
JournalJournal of Experimental Biology
Volume204
Issue number10
StatePublished - Dec 1 2001

Fingerprint

Oncorhynchus mykiss
Fresh Water
pavement
rainbow
Cultured Cells
gills
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Primary Cell Culture
Patch-Clamp Techniques
cells
diphenylamine
patch-clamp technique
Anions
Permeability
Fishes
freshwater fish
seals
anions
permeability

Keywords

  • 'Maxi' Cl channel
  • DIDS
  • Freshwater teleost
  • Gill
  • Oncorhynchus mykiss
  • Patch-clamp
  • Rainbow trout
  • SITS
  • Zn

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

A maxi Cl- channel in cultured pavement cells from the gills of the freshwater rainbow trout Oncorhynchus mykiss. / O'Donnell, M. J.; Kelly, S. P.; Nurse, C. A.; Wood, C. M.

In: Journal of Experimental Biology, Vol. 204, No. 10, 01.12.2001, p. 1783-1794.

Research output: Contribution to journalArticle

O'Donnell, M. J. ; Kelly, S. P. ; Nurse, C. A. ; Wood, C. M. / A maxi Cl- channel in cultured pavement cells from the gills of the freshwater rainbow trout Oncorhynchus mykiss. In: Journal of Experimental Biology. 2001 ; Vol. 204, No. 10. pp. 1783-1794.
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