A mammalian protein with specific demethylase activity for mCpG DNA

Sanjoy K. Bhattacharya; Shyam Ramchandani; Nadia Cervoni; Moshe Szyf

doi:10.1038/17533

A mammalian protein with specific demethylase activity for mCpG DNA

Sanjoy K. Bhattacharya, Shyam Ramchandani, Nadia Cervoni, Moshe Szyf

Research output: Contribution to journal › Article

525 Scopus citations

Abstract

DNA-methylation patterns are Important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

Original language	English (US)
Pages (from-to)	579-583
Number of pages	5
Journal	Nature
Volume	397
Issue number	6720
DOIs	https://doi.org/10.1038/17533
State	Published - Feb 18 1999
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/17533

Fingerprint Dive into the research topics of 'A mammalian protein with specific demethylase activity for mCpG DNA'. Together they form a unique fingerprint.

Cite this

Bhattacharya, S. K., Ramchandani, S., Cervoni, N., & Szyf, M. (1999). A mammalian protein with specific demethylase activity for mCpG DNA. Nature, 397(6720), 579-583. https://doi.org/10.1038/17533

@article{2154714920674ecb9cdab1de7db1e57d,

title = "A mammalian protein with specific demethylase activity for mCpG DNA",

abstract = "DNA-methylation patterns are Important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.",

author = "Bhattacharya, {Sanjoy K.} and Shyam Ramchandani and Nadia Cervoni and Moshe Szyf",

year = "1999",

month = feb,

day = "18",

doi = "10.1038/17533",

language = "English (US)",

volume = "397",

pages = "579--583",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "6720",

}

TY - JOUR

T1 - A mammalian protein with specific demethylase activity for mCpG DNA

AU - Bhattacharya, Sanjoy K.

AU - Ramchandani, Shyam

AU - Cervoni, Nadia

AU - Szyf, Moshe

PY - 1999/2/18

Y1 - 1999/2/18

N2 - DNA-methylation patterns are Important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

AB - DNA-methylation patterns are Important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

UR - http://www.scopus.com/inward/record.url?scp=0033580326&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033580326&partnerID=8YFLogxK

U2 - 10.1038/17533

DO - 10.1038/17533

M3 - Article

C2 - 10050851

AN - SCOPUS:0033580326

VL - 397

SP - 579

EP - 583

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6720

ER -