A licensing step links AID to transcription elongation for mutagenesis in B cells

Stephen P. Methot; Ludivine C. Litzler; Poorani Ganesh Subramani; Anil K. Eranki; Heather Fifield; Anne Marie Patenaude; Julian C. Gilmore; Gabriel E. Santiago; Halil Bagci; Jean François Côté; Mani Larijani; Ramiro E. Verdun; Javier M. Di Noia

doi:10.1038/s41467-018-03387-6

A licensing step links AID to transcription elongation for mutagenesis in B cells

Stephen P. Methot, Ludivine C. Litzler, Poorani Ganesh Subramani, Anil K. Eranki, Heather Fifield, Anne Marie Patenaude, Julian C. Gilmore, Gabriel E. Santiago, Halil Bagci, Jean François Côté, Mani Larijani, Ramiro E. Verdun, Javier M. Di Noia

Medicine

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

Activation-induced deaminase (AID) mutates the immunoglobulin (Ig) genes to initiate somatic hypermutation (SHM) and class switch recombination (CSR) in B cells, thus underpinning antibody responses. AID mutates a few hundred other loci, but most AID-occupied genes are spared. The mechanisms underlying productive deamination versus non-productive AID targeting are unclear. Here we show that three clustered arginine residues define a functional AID domain required for SHM, CSR, and off-target activity in B cells without affecting AID deaminase activity or Escherichia coli mutagenesis. Both wt AID and mutants with single amino acid replacements in this domain broadly associate with Spt5 and chromatin and occupy the promoter of AID target genes. However, mutant AID fails to occupy the corresponding gene bodies and loses association with transcription elongation factors. Thus AID mutagenic activity is determined not by locus occupancy but by a licensing mechanism, which couples AID to transcription elongation.

Original language	English (US)
Article number	1248
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03387-6
State	Published - Dec 1 2018

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Methot, S. P., Litzler, L. C., Subramani, P. G., Eranki, A. K., Fifield, H., Patenaude, A. M., Gilmore, J. C., Santiago, G. E., Bagci, H., Côté, J. F., Larijani, M., Verdun, R. E., & Di Noia, J. M. (2018). A licensing step links AID to transcription elongation for mutagenesis in B cells. Nature communications, 9(1), [1248]. https://doi.org/10.1038/s41467-018-03387-6

A licensing step links AID to transcription elongation for mutagenesis in B cells. / Methot, Stephen P.; Litzler, Ludivine C.; Subramani, Poorani Ganesh; Eranki, Anil K.; Fifield, Heather; Patenaude, Anne Marie; Gilmore, Julian C.; Santiago, Gabriel E.; Bagci, Halil; Côté, Jean François; Larijani, Mani; Verdun, Ramiro E.; Di Noia, Javier M.

In: Nature communications, Vol. 9, No. 1, 1248, 01.12.2018.

Research output: Contribution to journal › Article

Methot, Stephen P. ; Litzler, Ludivine C. ; Subramani, Poorani Ganesh ; Eranki, Anil K. ; Fifield, Heather ; Patenaude, Anne Marie ; Gilmore, Julian C. ; Santiago, Gabriel E. ; Bagci, Halil ; Côté, Jean François ; Larijani, Mani ; Verdun, Ramiro E. ; Di Noia, Javier M. / A licensing step links AID to transcription elongation for mutagenesis in B cells. In: Nature communications. 2018 ; Vol. 9, No. 1.

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