A licensing step links AID to transcription elongation for mutagenesis in B cells

Stephen P. Methot, Ludivine C. Litzler, Poorani Ganesh Subramani, Anil K. Eranki, Heather Fifield, Anne Marie Patenaude, Julian C. Gilmore, Gabriel E. Santiago, Halil Bagci, Jean François Côté, Mani Larijani, Ramiro E Verdun, Javier M. Di Noia

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Activation-induced deaminase (AID) mutates the immunoglobulin (Ig) genes to initiate somatic hypermutation (SHM) and class switch recombination (CSR) in B cells, thus underpinning antibody responses. AID mutates a few hundred other loci, but most AID-occupied genes are spared. The mechanisms underlying productive deamination versus non-productive AID targeting are unclear. Here we show that three clustered arginine residues define a functional AID domain required for SHM, CSR, and off-target activity in B cells without affecting AID deaminase activity or Escherichia coli mutagenesis. Both wt AID and mutants with single amino acid replacements in this domain broadly associate with Spt5 and chromatin and occupy the promoter of AID target genes. However, mutant AID fails to occupy the corresponding gene bodies and loses association with transcription elongation factors. Thus AID mutagenic activity is determined not by locus occupancy but by a licensing mechanism, which couples AID to transcription elongation.

Original languageEnglish (US)
Article number1248
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

mutagenesis
licensing
Mutagenesis
Transcription
Licensure
elongation
Elongation
B-Lymphocytes
Cells
activation
genes
Genes
loci
Genetic Recombination
AICDA (activation-induced cytidine deaminase)
switches
Switches
Peptide Elongation Factors
Immunoglobulin Genes
chromatin

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Methot, S. P., Litzler, L. C., Subramani, P. G., Eranki, A. K., Fifield, H., Patenaude, A. M., ... Di Noia, J. M. (2018). A licensing step links AID to transcription elongation for mutagenesis in B cells. Nature Communications, 9(1), [1248]. https://doi.org/10.1038/s41467-018-03387-6

A licensing step links AID to transcription elongation for mutagenesis in B cells. / Methot, Stephen P.; Litzler, Ludivine C.; Subramani, Poorani Ganesh; Eranki, Anil K.; Fifield, Heather; Patenaude, Anne Marie; Gilmore, Julian C.; Santiago, Gabriel E.; Bagci, Halil; Côté, Jean François; Larijani, Mani; Verdun, Ramiro E; Di Noia, Javier M.

In: Nature Communications, Vol. 9, No. 1, 1248, 01.12.2018.

Research output: Contribution to journalArticle

Methot, SP, Litzler, LC, Subramani, PG, Eranki, AK, Fifield, H, Patenaude, AM, Gilmore, JC, Santiago, GE, Bagci, H, Côté, JF, Larijani, M, Verdun, RE & Di Noia, JM 2018, 'A licensing step links AID to transcription elongation for mutagenesis in B cells', Nature Communications, vol. 9, no. 1, 1248. https://doi.org/10.1038/s41467-018-03387-6
Methot SP, Litzler LC, Subramani PG, Eranki AK, Fifield H, Patenaude AM et al. A licensing step links AID to transcription elongation for mutagenesis in B cells. Nature Communications. 2018 Dec 1;9(1). 1248. https://doi.org/10.1038/s41467-018-03387-6
Methot, Stephen P. ; Litzler, Ludivine C. ; Subramani, Poorani Ganesh ; Eranki, Anil K. ; Fifield, Heather ; Patenaude, Anne Marie ; Gilmore, Julian C. ; Santiago, Gabriel E. ; Bagci, Halil ; Côté, Jean François ; Larijani, Mani ; Verdun, Ramiro E ; Di Noia, Javier M. / A licensing step links AID to transcription elongation for mutagenesis in B cells. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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