A keratin biomaterial gel hemostat derived from human hair: Evaluation in a rabbit model of lethal liver injury

Tamer Aboushwareb, Daniel Eberli, Catherine Ward, Christopher Broda, John Holcomb, Anthony Atala, Mark Van Dyke

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Effective hemostatic dressings that are compatible with tissues are needed. Keratins are a class of biomaterials that can be derived by extraction of proteins from human hair. We have recently discovered that keratin biomaterials have hemostatic characteristics and hypothesize that a keratin hydrogel having the ability to absorb fluid and bind cells may be an effective hemostat. The goal of this study was to test a keratin hydrogel and evaluate it compared to current hemostats. Thirty-two New Zealand white rabbits received a lethal liver injury. Eight animals each were assigned to negative control, QuickClot®, HemCon® bandage, and keratin treatment groups. Vital stats and other data were recorded during surgery and all surviving animals were sacrificed after 72 h. Histology was conducted on all surviving animals. Twenty-four-hour survival rates were 0%, 62.5%, 62.5%, and 75% for the negative control, QuickClot, HemCon, and keratin groups, respectively. Other outcomes included blood loss, mean arterial pressure, heart rate, shock index, and liver histology. All of the hemostats were statistically better than the negative control group at late operative time points. The keratin group consistently performed as well as, or better than, the commercial hemostats. Histology showed an interesting healing response at the hemostat-liver interface in the keratin group.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume90
Issue number1
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

Fingerprint

Keratin
Biocompatible Materials
Keratins
Biomaterials
Liver
Gels
Histology
Animals
Hydrogel
Hemostatics
Hydrogels
Surgery
Blood
Tissue
Proteins
Fluids

Keywords

  • Hemorrhage
  • Hemostasis
  • Hemostat
  • Keratin
  • Liver injury

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

A keratin biomaterial gel hemostat derived from human hair : Evaluation in a rabbit model of lethal liver injury. / Aboushwareb, Tamer; Eberli, Daniel; Ward, Catherine; Broda, Christopher; Holcomb, John; Atala, Anthony; Van Dyke, Mark.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 90, No. 1, 01.07.2009, p. 45-54.

Research output: Contribution to journalArticle

Aboushwareb, Tamer ; Eberli, Daniel ; Ward, Catherine ; Broda, Christopher ; Holcomb, John ; Atala, Anthony ; Van Dyke, Mark. / A keratin biomaterial gel hemostat derived from human hair : Evaluation in a rabbit model of lethal liver injury. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2009 ; Vol. 90, No. 1. pp. 45-54.
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