A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus

Diogo M. Magnani; Cassia G.T. Silveira; Brandon C. Rosen; Michael J. Ricciardi; Núria Pedreño-Lopez; Martin J. Gutman; Varian K. Bailey; Helen S. Maxwell; Aline Domingues; Lucas Gonzalez-Nieto; Vivian I. Avelino-Silva; Mateus Trindade; Juliana Nogueira; Consuelo S. Oliveira; Alvino Maestri; Alvina Clara Felix; José Eduardo Levi; Mauricio L. Nogueira; Mauricio A. Martins; José M. Martinez-Navio; Sebastian P. Fuchs; Stephen S. Whitehead; Dennis R. Burton; Ronald C. Desrosiers; Esper G. Kallas; David I. Watkins

doi:10.1371/journal.pntd.0005655

A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus

Diogo M. Magnani, Cassia G.T. Silveira, Brandon C. Rosen, Michael J. Ricciardi, Núria Pedreño-Lopez, Martin J. Gutman, Varian K. Bailey, Helen S. Maxwell, Aline Domingues, Lucas Gonzalez-Nieto, Vivian I. Avelino-Silva, Mateus Trindade, Juliana Nogueira, Consuelo S. Oliveira, Alvino Maestri, Alvina Clara Felix, José Eduardo Levi, Mauricio L. Nogueira, Mauricio A. Martins, José M. Martinez-NavioSebastian P. Fuchs, Stephen S. Whitehead, Dennis R. Burton, Ronald C. Desrosiers, Esper G. Kallas, David I. Watkins

Pathology

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut₅₀~ 2 μg/ml) but did not react with any of the four dengue virus serotypes. Except for the expected junctional diversity created by the joining of the V-(D)-J genes, there was no deviation from immunoglobulin germline genes. This is a rare example of a human mAb with neutralizing activity in the absence of detectable somatic hypermutation. Importantly, binding of this mAb to ZIKV was specifically inhibited by human plasma from ZIKV-exposed individuals, suggesting that it may be of value in a diagnostic setting.

Original language	English (US)
Article number	e0005655
Journal	PLoS neglected tropical diseases
Volume	11
Issue number	6
DOIs	https://doi.org/10.1371/journal.pntd.0005655
State	Published - Jun 12 2017

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1371/journal.pntd.0005655

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Magnani, D. M., Silveira, C. G. T., Rosen, B. C., Ricciardi, M. J., Pedreño-Lopez, N., Gutman, M. J., Bailey, V. K., Maxwell, H. S., Domingues, A., Gonzalez-Nieto, L., Avelino-Silva, V. I., Trindade, M., Nogueira, J., Oliveira, C. S., Maestri, A., Felix, A. C., Levi, J. E., Nogueira, M. L., Martins, M. A., ... Watkins, D. I. (2017). A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. PLoS neglected tropical diseases, 11(6), [e0005655]. https://doi.org/10.1371/journal.pntd.0005655

A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. / Magnani, Diogo M.; Silveira, Cassia G.T.; Rosen, Brandon C.; Ricciardi, Michael J.; Pedreño-Lopez, Núria; Gutman, Martin J.; Bailey, Varian K.; Maxwell, Helen S.; Domingues, Aline; Gonzalez-Nieto, Lucas; Avelino-Silva, Vivian I.; Trindade, Mateus; Nogueira, Juliana; Oliveira, Consuelo S.; Maestri, Alvino; Felix, Alvina Clara; Levi, José Eduardo; Nogueira, Mauricio L.; Martins, Mauricio A.; Martinez-Navio, José M.; Fuchs, Sebastian P.; Whitehead, Stephen S.; Burton, Dennis R.; Desrosiers, Ronald C.; Kallas, Esper G.; Watkins, David I.

In: PLoS neglected tropical diseases, Vol. 11, No. 6, e0005655, 12.06.2017.

Research output: Contribution to journal › Article

Magnani, DM, Silveira, CGT, Rosen, BC, Ricciardi, MJ, Pedreño-Lopez, N, Gutman, MJ, Bailey, VK, Maxwell, HS, Domingues, A, Gonzalez-Nieto, L, Avelino-Silva, VI, Trindade, M, Nogueira, J, Oliveira, CS, Maestri, A, Felix, AC, Levi, JE, Nogueira, ML, Martins, MA, Martinez-Navio, JM, Fuchs, SP, Whitehead, SS, Burton, DR, Desrosiers, RC, Kallas, EG & Watkins, DI 2017, 'A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus', PLoS neglected tropical diseases, vol. 11, no. 6, e0005655. https://doi.org/10.1371/journal.pntd.0005655

Magnani, Diogo M. ; Silveira, Cassia G.T. ; Rosen, Brandon C. ; Ricciardi, Michael J. ; Pedreño-Lopez, Núria ; Gutman, Martin J. ; Bailey, Varian K. ; Maxwell, Helen S. ; Domingues, Aline ; Gonzalez-Nieto, Lucas ; Avelino-Silva, Vivian I. ; Trindade, Mateus ; Nogueira, Juliana ; Oliveira, Consuelo S. ; Maestri, Alvino ; Felix, Alvina Clara ; Levi, José Eduardo ; Nogueira, Mauricio L. ; Martins, Mauricio A. ; Martinez-Navio, José M. ; Fuchs, Sebastian P. ; Whitehead, Stephen S. ; Burton, Dennis R. ; Desrosiers, Ronald C. ; Kallas, Esper G. ; Watkins, David I. / A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus. In: PLoS neglected tropical diseases. 2017 ; Vol. 11, No. 6.

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