A hierarchy of gene expression accompanying induction of the primitive streak by Vg1 in the chick embryo

Isaac Skromne, Claudio D. Stern

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

In the chick embryo, two secreted factors have recently been shown to cooperate in inducing the first axial structure, the primitive streak: cWnt8C (normally expressed around the circumference of the embryo, in the marginal zone) and the TGFβ superfamily member cVg1 (expressed in the posterior part of the marginal zone) (Development 128 (2001) 2915). Misexpression of Vg1 in the anterior marginal zone induces an ectopic primitive streak and recapitulates the morphological changes associated with normal primitive streak formation. Here, we analyse the time-course of appearance and disappearance of expression of 12 genes (cVg1, Lef1, Nodal, FGF8, cWnt8C, cBra, cNot1, goosecoid, HNF3β, Chordin, Otx2 and Sox3, whose normal expression is also polarized at early stages of development) in response to cVg1 misexpression in the anterior marginal zone. We show that a hierarchy of gene expression accompanies induction of the ectopic axis, reminiscent of the order in which the same genes begin to be expressed in the normal embryo.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalMechanisms of Development
Volume114
Issue number1-2
DOIs
StatePublished - May 13 2002

Keywords

  • cBrachyury
  • cFGF8
  • Chicken embryo
  • Chordin
  • cLef1
  • cNodal
  • cNot1
  • cVg1
  • Gastrulation
  • Goosecoid
  • Hensen's node
  • HNF3β
  • Induction
  • Otx-2
  • Primitive streak
  • Sox3
  • Spemann organizer
  • Wnt8C

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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