A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine

Anton Wellstein, Wenjing Fang, Ashok Khatri, Yang Lu, Sandra S. Swain, Robert B. Dickson, Joachim Sasse, Anna Tate Riegel, Marc E Lippman

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

We report purification of an 18-kDa heparin-binding growth factor secreted from human cancer cells which is homologous to a developmentally regulated, neurotrophic factor, heparin-binding growth-associated molecule/pleiotrophin (HB-GAM/PTN; Merenmies, J., and Rauvala, H. (1990) J. Biol. Chem. 265, 16721-16724; Li, Y. S., Milner, P. G., Chauhan, A. K., Watson, M. A., Hoffman, R. M., Kodner, C. M., Milbrandt, J., and Deuel, T. F. (1990) Science 250, 1690-1694). We have purified the protein from tissue culture supernatants of human breast cancer cells (MDA-MB 231) and have used soft agar cloning of an epithelial cell line (SW-13) to detect its growth stimulating activity. A 32,000-fold purification was achieved by isoelectric focusing, heparin affinity chromatography, and reversed phase high pressure liquid chromatography. The molecular mass of the protein was confirmed by gel filtration chromatography in the presence of detergent and bioassay of the fractions. The N-terminal sequence was homologous to HB-GAM/PTN, and polymerase chain reaction amplification and DNA sequencing confirmed that the respective transcript was present in the cancer cells. We conclude that HB-GAM/ PTN can function as a tumor growth factor in addition to its role during neuronal development.

Original languageEnglish
Pages (from-to)2582-2587
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number4
StatePublished - Feb 5 1992
Externally publishedYes

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Heparin
Intercellular Signaling Peptides and Proteins
Cells
Breast Neoplasms
Cytokines
Purification
High pressure liquid chromatography
Affinity chromatography
Neoplasms
Tissue culture
Bioassay
Cloning
Polymerase chain reaction
Nerve Growth Factors
Isoelectric Focusing
Molecular mass
Reverse-Phase Chromatography
Sequence Homology
Chromatography
DNA Sequence Analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wellstein, A., Fang, W., Khatri, A., Lu, Y., Swain, S. S., Dickson, R. B., ... Lippman, M. E. (1992). A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine. Journal of Biological Chemistry, 267(4), 2582-2587.

A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine. / Wellstein, Anton; Fang, Wenjing; Khatri, Ashok; Lu, Yang; Swain, Sandra S.; Dickson, Robert B.; Sasse, Joachim; Riegel, Anna Tate; Lippman, Marc E.

In: Journal of Biological Chemistry, Vol. 267, No. 4, 05.02.1992, p. 2582-2587.

Research output: Contribution to journalArticle

Wellstein, A, Fang, W, Khatri, A, Lu, Y, Swain, SS, Dickson, RB, Sasse, J, Riegel, AT & Lippman, ME 1992, 'A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine', Journal of Biological Chemistry, vol. 267, no. 4, pp. 2582-2587.
Wellstein, Anton ; Fang, Wenjing ; Khatri, Ashok ; Lu, Yang ; Swain, Sandra S. ; Dickson, Robert B. ; Sasse, Joachim ; Riegel, Anna Tate ; Lippman, Marc E. / A heparin-binding growth factor secreted from breast cancer cells homologous to a developmentally regulated cytokine. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 4. pp. 2582-2587.
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