A hammerhead ribozyme that cleaves perforin and fas-ligand RNAs in vitro

Zhimei Du, Camillo Ricordi, Eckhard R Podack, Ricardo L. Pastori

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Polyfunctional ribozymes targeting more than one RNA might be ideal for treatment of diseases caused by the expression of more than one mRNA. This is the case of Graft versus Host Disease, which in the mouse model is due to the action of two proteins responsible for different lytic pathways: perforin and fas-ligand. We have created a bifunctional ribozyme fusing two antiperforin and antifas-ligand hammerhead ribozymes with a stretch of CA dinucleotides. This bifunctional ribozyme is able to recognize and cleave in vitro perforin and fas-ligand mRNA in a specific and selective fashion, with a catalytic efficiency similar to that of its individual components.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalBiochemical and biophysical research communications
Volume226
Issue number3
DOIs
StatePublished - Sep 24 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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