TY - JOUR
T1 - A guide to studying human Hair follicle cycling in vivo
AU - Oh, Ji Won
AU - Kloepper, Jennifer
AU - Langan, Ewan A.
AU - Kim, Yongsoo
AU - Yeo, Joongyeub
AU - Kim, Min Ji
AU - Hsi, Tsai Ching
AU - Rose, Christian
AU - Yoon, Ghil Suk
AU - Lee, Seok Jong
AU - Seykora, John
AU - Kim, Jung Chul
AU - Sung, Young Kwan
AU - Kim, Moonkyu
AU - Paus, Ralf
AU - Plikus, Maksim V.
N1 - Funding Information:
JWO is supported by the Regenerative Medicine R&D fund provided by Daegu city (Korea), and ETRI R&D Program (15ZC3100). MVP is supported by the NIH National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) grant R01-AR067273, Edward Mallinckrodt Jr. Foundation grant, and the University of California Cancer Research Coordinating Committee (CRCC) grant. This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2012R1A1B3001047 to YKS and NRF-2014R1A5A2009242 to MK), and by a grant from Deutsche Forschungsgemeinschaft (Pa 345/13-1) to RP. This research was also supported by Kyungpook National University Research Fund, 2012. The authors are most grateful and indebted to many colleagues, who generously lent a helping hand and/or provided expert advice during some stage of this long-lasting expedition into human hair cycle staging, in particular to Sang In Choi, Chang Hoon Seo, Mi Hee Kwack, Seung Hyun Shin, Sanguk Im, Jin Oh Kim, Dorothee Langan, Koji Sugawara, Nadine Dörwald, Jonathan Le, Manda Nguyen, David Whiting and George Cotsarelis.
Publisher Copyright:
© 2015 The Authors. Published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology.
PY - 2016/1
Y1 - 2016/1
N2 - Hair follicles (HFs) undergo lifelong cyclical transformations, progressing through stages of rapid growth (anagen), regression (catagen), and relative "quiescence" (telogen). Given that HF cycling abnormalities underlie many human hair growth disorders, the accurate classification of individual cycle stages within skin biopsies is clinically important and essential for hair research. For preclinical human hair research purposes, human scalp skin can bexenografted onto immunocompromised mice tostudy human HF cyclingandmanipulate long-lasting anagen in vivo. Although available for mice, a comprehensive guide on how to recognize different human hair cycle stages in vivo is lacking. In this article, we present such a guide, which uses objective, well-defined, and reproducible criteria, and integrates simple morphological indicators with advanced, (immuno)-histochemical markers. This guide also characterizes human HF cycling in xenografts and highlights the utility of this model for in vivo hair research. Detailed schematic drawings and representative micrographs provide examples of how best to identify human HF stages, even in suboptimally sectioned tissue, and practical recommendations are given for designing human-on-mouse hair cycle experiments. Thus, this guide seeks to offer a benchmark for human hair cycle stage classification, for both hair research experts and newcomers to the field.
AB - Hair follicles (HFs) undergo lifelong cyclical transformations, progressing through stages of rapid growth (anagen), regression (catagen), and relative "quiescence" (telogen). Given that HF cycling abnormalities underlie many human hair growth disorders, the accurate classification of individual cycle stages within skin biopsies is clinically important and essential for hair research. For preclinical human hair research purposes, human scalp skin can bexenografted onto immunocompromised mice tostudy human HF cyclingandmanipulate long-lasting anagen in vivo. Although available for mice, a comprehensive guide on how to recognize different human hair cycle stages in vivo is lacking. In this article, we present such a guide, which uses objective, well-defined, and reproducible criteria, and integrates simple morphological indicators with advanced, (immuno)-histochemical markers. This guide also characterizes human HF cycling in xenografts and highlights the utility of this model for in vivo hair research. Detailed schematic drawings and representative micrographs provide examples of how best to identify human HF stages, even in suboptimally sectioned tissue, and practical recommendations are given for designing human-on-mouse hair cycle experiments. Thus, this guide seeks to offer a benchmark for human hair cycle stage classification, for both hair research experts and newcomers to the field.
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U2 - 10.1038/JID.2015.354
DO - 10.1038/JID.2015.354
M3 - Article
C2 - 26763421
AN - SCOPUS:84959526189
VL - 136
SP - 34
EP - 44
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 1
ER -