A guide to studying human dermal adipocytes in situ

Carina Nicu, Jennifer Pople, Laura Bonsell, Ranjit Bhogal, David M. Ansell, Ralf Paus

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Dermal white adipose tissue (DWAT) is a main component of human skin, composed of individual lipid-laden mesenchymal cells known as dermal adipocytes (DAs). Besides their well-known role in lipid storage and release, DAs also promote skin immunity, wound healing and hair follicle cycling and are important players in cutaneous neuroendocrinology. The ever-growing insights into DWAT functions, albeit mostly in mice, have invited speculation that it may be involved in multiple skin diseases ranging from fibrosis to alopecia and psoriasis, thus designating human DWAT a clinically relevant, but as yet insufficiently investigated skin compartment. Therefore, this practical, user-friendly guide aims to introduce the techniques available to study human DWAT in situ and ex vivo, including immunohistochemistry, immunofluorescence microscopy and analysis via quantitative immunohistomorphometry. Here, we provide information on a collection of stains comprising pre-adipocyte (Pref1) and mature adipocyte markers (Perilipin1, Caveolin1), as well as various lipid (OilRedO, BODIPY) and histochemical stains (H&E, trichrome) available for use on human DWAT. We offer the reader guidelines on fixing, processing and staining human DAs and highlight caveats and solutions to common problems that one may encounter when studying this fascinating skin compartment. We also suggest standard methods for conducting quantitative immunohistomorphometry on human DWAT and its individual adipocytes to quantify cell size, number, lipid content and fluorescence intensity of adipose-specific markers. Finally, we briefly introduce in situ hybridization, transmission electron microscopy and essentials of magnetic resonance imaging imaging as additional tools for instructively interrogating this largest, but still least-known compartment of human skin.

Original languageEnglish (US)
Pages (from-to)589-602
Number of pages14
JournalExperimental Dermatology
Volume27
Issue number6
DOIs
StatePublished - Jun 1 2018
Externally publishedYes

Fingerprint

Adipocytes
Skin
Tissue
White Adipose Tissue
Lipids
Coloring Agents
Imaging techniques
Magnetic resonance
Microscopic examination
Fluorescence
Transmission electron microscopy
Neuroendocrinology
Processing
Hair Follicle
Alopecia
Transmission Electron Microscopy
Cell Size
Fluorescence Microscopy
Psoriasis
Skin Diseases

Keywords

  • immunofluorescence
  • immunohistochemistry
  • white adipose tissue

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Nicu, C., Pople, J., Bonsell, L., Bhogal, R., Ansell, D. M., & Paus, R. (2018). A guide to studying human dermal adipocytes in situ. Experimental Dermatology, 27(6), 589-602. https://doi.org/10.1111/exd.13549

A guide to studying human dermal adipocytes in situ. / Nicu, Carina; Pople, Jennifer; Bonsell, Laura; Bhogal, Ranjit; Ansell, David M.; Paus, Ralf.

In: Experimental Dermatology, Vol. 27, No. 6, 01.06.2018, p. 589-602.

Research output: Contribution to journalReview article

Nicu, C, Pople, J, Bonsell, L, Bhogal, R, Ansell, DM & Paus, R 2018, 'A guide to studying human dermal adipocytes in situ', Experimental Dermatology, vol. 27, no. 6, pp. 589-602. https://doi.org/10.1111/exd.13549
Nicu C, Pople J, Bonsell L, Bhogal R, Ansell DM, Paus R. A guide to studying human dermal adipocytes in situ. Experimental Dermatology. 2018 Jun 1;27(6):589-602. https://doi.org/10.1111/exd.13549
Nicu, Carina ; Pople, Jennifer ; Bonsell, Laura ; Bhogal, Ranjit ; Ansell, David M. ; Paus, Ralf. / A guide to studying human dermal adipocytes in situ. In: Experimental Dermatology. 2018 ; Vol. 27, No. 6. pp. 589-602.
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