A frameshift mutation at the NH2 terminus of the nucleoprotein gene does not affect generation of cytotoxic T lymphocyte epitopes

James V. Fetten, Nita Roy, Eli Gilboa

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

BALB/3T3 cells infected with a retroviral vector encoding the influenza virus nucleoprotein (NP) gene are efficiently lysed by CTL generated in BALB/c mice (H-2d background). Cells transduced with a mutant form of NP which contains a frameshift mutation at its NH2 terminus (NPm) do not express biochemically detectable levels of protein but nevertheless present Ag to CTL with high efficiency. Cold target inhibition studies indicate that the same CTL epitope(s) are recognized in cells harboring NP or NPm. L929 cells transduced with the NPm gene also present Ag efficiently to CTL raised in C3H mice (H-2k background). Cells engineered to express 5- to 15-fold lower levels of wild-type NP were not capable of presenting Ag to CTL, arguing against the notion that CTL are able to lyse cells expressing very low levels of Ag which might have resulted from suppression of the frameshift mutation in NPm. Implications to the mechanism of epitope generation in class I MHC-restricted immune responses are considered.

Original languageEnglish
Pages (from-to)2697-2705
Number of pages9
JournalJournal of Immunology
Volume147
Issue number8
StatePublished - Oct 15 1991
Externally publishedYes

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Frameshift Mutation
Nucleoproteins
T-Lymphocyte Epitopes
Cytotoxic T-Lymphocytes
Genes
Epitopes
BALB 3T3 Cells
Inbred C3H Mouse
Orthomyxoviridae
Proteins

ASJC Scopus subject areas

  • Immunology

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A frameshift mutation at the NH2 terminus of the nucleoprotein gene does not affect generation of cytotoxic T lymphocyte epitopes. / Fetten, James V.; Roy, Nita; Gilboa, Eli.

In: Journal of Immunology, Vol. 147, No. 8, 15.10.1991, p. 2697-2705.

Research output: Contribution to journalArticle

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