A force-based protein biochip

K. Blank, T. Mai, I. Gilbert, S. Schiffmann, J. Rankl, R. Zivin, C. Tackney, T. Nicolaus, K. Spinnler, F. Oesterhelt, M. Benoit, H. Clausen-Schaumann, H. E. Gaub

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand-receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein-protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.

Original languageEnglish
Pages (from-to)11356-11360
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number20
DOIs
StatePublished - Sep 30 2003

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Protein Array Analysis
Antibodies
DNA
Hepatitis C Antigens
Proteins
Ligands

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Blank, K., Mai, T., Gilbert, I., Schiffmann, S., Rankl, J., Zivin, R., ... Gaub, H. E. (2003). A force-based protein biochip. Proceedings of the National Academy of Sciences of the United States of America, 100(20), 11356-11360. https://doi.org/10.1073/pnas.1934928100

A force-based protein biochip. / Blank, K.; Mai, T.; Gilbert, I.; Schiffmann, S.; Rankl, J.; Zivin, R.; Tackney, C.; Nicolaus, T.; Spinnler, K.; Oesterhelt, F.; Benoit, M.; Clausen-Schaumann, H.; Gaub, H. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 20, 30.09.2003, p. 11356-11360.

Research output: Contribution to journalArticle

Blank, K, Mai, T, Gilbert, I, Schiffmann, S, Rankl, J, Zivin, R, Tackney, C, Nicolaus, T, Spinnler, K, Oesterhelt, F, Benoit, M, Clausen-Schaumann, H & Gaub, HE 2003, 'A force-based protein biochip', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 20, pp. 11356-11360. https://doi.org/10.1073/pnas.1934928100
Blank K, Mai T, Gilbert I, Schiffmann S, Rankl J, Zivin R et al. A force-based protein biochip. Proceedings of the National Academy of Sciences of the United States of America. 2003 Sep 30;100(20):11356-11360. https://doi.org/10.1073/pnas.1934928100
Blank, K. ; Mai, T. ; Gilbert, I. ; Schiffmann, S. ; Rankl, J. ; Zivin, R. ; Tackney, C. ; Nicolaus, T. ; Spinnler, K. ; Oesterhelt, F. ; Benoit, M. ; Clausen-Schaumann, H. ; Gaub, H. E. / A force-based protein biochip. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 20. pp. 11356-11360.
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