A fluorometric method for the determination of triglycerides in nanomolar quantities

Armando J Mendez, Carmen Cabeza, S. L. Hsia

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.

Original languageEnglish
Pages (from-to)386-389
Number of pages4
JournalAnalytical Biochemistry
Volume156
Issue number2
DOIs
StatePublished - Aug 1 1986

Fingerprint

glycerol-3-phosphate oxidase
Triglycerides
Glycerol Kinase
Triolein
Assays
Lipase
Glycerol
Peroxidase
Wavelength
4-hydroxyphenylacetic acid
alpha-glycerophosphoric acid

Keywords

  • fluorometric assay
  • glycerol-3-phosphate oxidase
  • lipase
  • triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A fluorometric method for the determination of triglycerides in nanomolar quantities. / Mendez, Armando J; Cabeza, Carmen; Hsia, S. L.

In: Analytical Biochemistry, Vol. 156, No. 2, 01.08.1986, p. 386-389.

Research output: Contribution to journalArticle

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