A fluorometric method for the determination of triglycerides in nanomolar quantities

Armando J. Mendez; Carmen Cabeza; S. L. Hsia

doi:10.1016/0003-2697(86)90269-1

A fluorometric method for the determination of triglycerides in nanomolar quantities

Armando J. Mendez, Carmen Cabeza, S. L. Hsia

Medicine

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H₂O₂. The H₂O₂ ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.

Original language	English (US)
Pages (from-to)	386-389
Number of pages	4
Journal	Analytical Biochemistry
Volume	156
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(86)90269-1
State	Published - Aug 1 1986

Keywords

fluorometric assay
glycerol-3-phosphate oxidase
lipase
triglycerides

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(86)90269-1

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title = "A fluorometric method for the determination of triglycerides in nanomolar quantities",

abstract = "A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.",

keywords = "fluorometric assay, glycerol-3-phosphate oxidase, lipase, triglycerides",

author = "Mendez, {Armando J.} and Carmen Cabeza and Hsia, {S. L.}",

note = "Funding Information: This work was supported in part by funds from the Dermatology Foundation of Miami and a grant from the Wallace Genetic Foundation. C.C. was a recipient of a MARC Scholarship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

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T1 - A fluorometric method for the determination of triglycerides in nanomolar quantities

AU - Mendez, Armando J.

AU - Cabeza, Carmen

AU - Hsia, S. L.

N1 - Funding Information: This work was supported in part by funds from the Dermatology Foundation of Miami and a grant from the Wallace Genetic Foundation. C.C. was a recipient of a MARC Scholarship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/8/1

Y1 - 1986/8/1

N2 - A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.

AB - A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.

KW - fluorometric assay

KW - glycerol-3-phosphate oxidase

KW - lipase

KW - triglycerides

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