A fluorometric method for the determination of triglycerides in nanomolar quantities

Armando J. Mendez, Carmen Cabeza, S. L. Hsia

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.

Original languageEnglish (US)
Pages (from-to)386-389
Number of pages4
JournalAnalytical Biochemistry
Volume156
Issue number2
DOIs
StatePublished - Aug 1 1986

Keywords

  • fluorometric assay
  • glycerol-3-phosphate oxidase
  • lipase
  • triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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