Abstract
A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.
| Original language | English |
|---|---|
| Pages (from-to) | 386-389 |
| Number of pages | 4 |
| Journal | Analytical Biochemistry |
| Volume | 156 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 1986 |
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Keywords
- fluorometric assay
- glycerol-3-phosphate oxidase
- lipase
- triglycerides
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A fluorometric method for the determination of triglycerides in nanomolar quantities. / Mendez, Armando J; Cabeza, Carmen; Hsia, S. L.
In: Analytical Biochemistry, Vol. 156, No. 2, 01.08.1986, p. 386-389.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A fluorometric method for the determination of triglycerides in nanomolar quantities
AU - Mendez, Armando J
AU - Cabeza, Carmen
AU - Hsia, S. L.
PY - 1986/8/1
Y1 - 1986/8/1
N2 - A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.
AB - A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.
KW - fluorometric assay
KW - glycerol-3-phosphate oxidase
KW - lipase
KW - triglycerides
UR - http://www.scopus.com/inward/record.url?scp=0022505735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022505735&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(86)90269-1
DO - 10.1016/0003-2697(86)90269-1
M3 - Article
C2 - 3766939
AN - SCOPUS:0022505735
VL - 156
SP - 386
EP - 389
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -