Abstract
A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 386-389 |
| Number of pages | 4 |
| Journal | Analytical Biochemistry |
| Volume | 156 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 1986 |
Keywords
- fluorometric assay
- glycerol-3-phosphate oxidase
- lipase
- triglycerides
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Mendez, A. J., Cabeza, C., & Hsia, S. L. (1986). A fluorometric method for the determination of triglycerides in nanomolar quantities. Analytical Biochemistry, 156(2), 386-389. https://doi.org/10.1016/0003-2697(86)90269-1