Abstract
A fluorometric assay for triglycerides in nanomole quantities is described. Glycerol is liberated from triglycerides with lipase from Chromobacter viscosum, then converted by glycerol kinase to glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase, producing H2O2. The H2O2 ultimately forms a peroxidase-catalyzed fluorogen with p-hydroxyphenylacetic acid. The excitation and emission wavelengths of the fluorogen are 325 and 415 nm, respectively. The assay is linear in the range 0.05-35 nmol of triglycerides using triolein as standard.
Original language | English (US) |
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Pages (from-to) | 386-389 |
Number of pages | 4 |
Journal | Analytical Biochemistry |
Volume | 156 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1 1986 |
Keywords
- fluorometric assay
- glycerol-3-phosphate oxidase
- lipase
- triglycerides
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology