A faster plant stem-water extraction method

Patricia F. Vendramini, Leonel Sternberg

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Oxygen and hydrogen isotope ratios of stem water have been used by several studies which relate the ecophysiology of plants to their water source. Undoubtedly, there are several other applications and research areas which could use this type of analysis. However, the most often used methods of extracting stem water are slow, limiting the rate of sampling and consequently preventing a deeper understanding of spatial and temporal plant water source use. We have developed a faster batch method of stem-water extraction and compare it with the most commonly used online method of stem-water extraction. Samples are sealed in 18 cm long ampoules having their extremities placed sample end in a heating block and the condensing end in a cooling block, and allowed to distill overnight. Up to 72 samples can be distilled overnight and sealed the next morning. The isotope ratios of water distilled by the batch method introduced here compared with those from the online method were in excellent agreement. In addition to being faster, this method does not need the monitoring of hot water baths and liquid nitrogen traps during distillation and does not require a complex vacuum system.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume21
Issue number2
DOIs
StatePublished - Jan 19 2007

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Water
Isotopes
Liquid nitrogen
Distillation
Hydrogen
Vacuum
Oxygen
Sampling
Cooling
Heating
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

A faster plant stem-water extraction method. / Vendramini, Patricia F.; Sternberg, Leonel.

In: Rapid Communications in Mass Spectrometry, Vol. 21, No. 2, 19.01.2007, p. 164-168.

Research output: Contribution to journalArticle

Vendramini, Patricia F. ; Sternberg, Leonel. / A faster plant stem-water extraction method. In: Rapid Communications in Mass Spectrometry. 2007 ; Vol. 21, No. 2. pp. 164-168.
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