A direct regulatory role for troponin T and a dual role for troponin C in the Ca2+ regulation of muscle contraction

James D. Potter, Zelin Sheng, Bo Sheng Pan, Jiaju Zhao

Research output: Contribution to journalArticle

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Abstract

Troponin (Tn), containing three subunits: Ca2+ binding (TnC), inhibitory (TnI), and tropomyosin binding (TnT), plays a crucial role in the Ca2+ regulation of vertebrate striated muscle contraction. These three subunits function by interacting with each other and with the other thin filament proteins. Previous studies suggested that the primary role of TnT is to anchor the TnI·TnC complex to the thin filament, primarily through its interactions with TnI and tropomyosin. We propose here a new role for TnT. Our results indicate that, when TnT is combined with the TnI·TnC complex, there is an activation of actomyosin ATPase that is Ca2+-dependent. To determine whether the latter results from a direct effect of TnC on TnT or indirectly from an effect of TnC on TnI which is transmitted to TnT, we prepared a deletion mutant (deletion of residues 1-57) of TnI, TnId57 (Sheng et al. (1992) J. Biol. Chem. 267, 25407-25413), which interacts with TnC but not TnT. Both wild type (TnI·TnC·TnT) and mutant (TnId57·TnC·TnT) Tn complexes demonstrated equivalent activity in the Ca2+ regulation of actomyosin-S1 ATPase activity. Similarly, both TnI and TnId57 could equally reconstitute TnI-depleted skinned muscle fibers. Therefore, since TnId57 does not interact with TnT, these results suggest that TnT reconstitutes native Ca2+ sensitivity via direct interaction with TnC. Thus Ca2+ binding to TnC would have a dual role: 1) release of the ATPase inhibition by TnI and 2) activation of the ATPase through interaction with TnT.

Original languageEnglish
Pages (from-to)2557-2562
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number6
StatePublished - Feb 10 1995
Externally publishedYes

Fingerprint

Troponin C
Tropomyosin
Troponin T
Muscle Contraction
Muscle
Adenosine Triphosphatases
Troponin
Myosins
Chemical activation
Actomyosin
Striated Muscle
Anchors
Vertebrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

A direct regulatory role for troponin T and a dual role for troponin C in the Ca2+ regulation of muscle contraction. / Potter, James D.; Sheng, Zelin; Pan, Bo Sheng; Zhao, Jiaju.

In: Journal of Biological Chemistry, Vol. 270, No. 6, 10.02.1995, p. 2557-2562.

Research output: Contribution to journalArticle

Potter, James D. ; Sheng, Zelin ; Pan, Bo Sheng ; Zhao, Jiaju. / A direct regulatory role for troponin T and a dual role for troponin C in the Ca2+ regulation of muscle contraction. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 6. pp. 2557-2562.
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