A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera

Timothy Cleary, A. Cid, B. Ellis, H. Malkus, T. Noto, S. Halbert, A. Castro

Research output: Contribution to journalArticle

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Abstract

A direct enzyme-linked immunoassay (ELISA), based on the 'sandwich' principle on an antigen-coated plastic disc, was used for the rapid detection of rubella antibody. Results were obtained the same day, and the prior adsorption of sera to remove non-specific inhibitors was not necessary. The ELISA was compared to the hemagglutination-inhibition (HAI) test on 500 serum samples. There was general agreement between the two methods; most discrepancies occurred with low-titered HAI positive sera. There was excellent correlation between the tests with serum samples negative for rubella antibodies and those samples with HAI titres ≥ 1:40.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume19
Issue number2
StatePublished - Dec 1 1978

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Rubella virus
Immunosorbents
Viruses
Assays
Enzyme-Linked Immunosorbent Assay
Antibodies
Hemagglutination
Enzymes
Serum
Plastics
Hemagglutination Inhibition Tests
Antigens
Adsorption
Immunoenzyme Techniques
rubella antibodies

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera. / Cleary, Timothy; Cid, A.; Ellis, B.; Malkus, H.; Noto, T.; Halbert, S.; Castro, A.

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 19, No. 2, 01.12.1978, p. 281-293.

Research output: Contribution to journalArticle

Cleary, Timothy ; Cid, A. ; Ellis, B. ; Malkus, H. ; Noto, T. ; Halbert, S. ; Castro, A. / A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera. In: Research Communications in Chemical Pathology and Pharmacology. 1978 ; Vol. 19, No. 2. pp. 281-293.
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