A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera

T. J. Cleary, A. Cid, B. Ellis, H. Malkus, T. Noto, S. Halbert, A. Castro

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A direct enzyme-linked immunoassay (ELISA), based on the 'sandwich' principle on an antigen-coated plastic disc, was used for the rapid detection of rubella antibody. Results were obtained the same day, and the prior adsorption of sera to remove non-specific inhibitors was not necessary. The ELISA was compared to the hemagglutination-inhibition (HAI) test on 500 serum samples. There was general agreement between the two methods; most discrepancies occurred with low-titered HAI positive sera. There was excellent correlation between the tests with serum samples negative for rubella antibodies and those samples with HAI titres ≥ 1:40.

Original languageEnglish (US)
Pages (from-to)281-293
Number of pages13
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume19
Issue number2
StatePublished - Dec 1 1978

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera'. Together they form a unique fingerprint.

  • Cite this