A device to facilitate limbal stem cell procurement from eye bank donor tissue for keratolimbal allograft procedures

David M. Meisler, Victor L Perez Quinones, James Proudfit

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To develop a device that facilitates the procurement of corneal limbal stem cell grafts for keratolimbal allograft procedures used in the treatment of ocular surface disease associated with stem cell deficiency. Description of device design and technique for use. The device is composed of a pedestal with a convex surface mounted to a flat platform. A corneoscleral button placed endothelial side down and centrally upon the convexity is secured by suction conveyed through a hollowed core in the pedestal that connects to fenestrated openings on the convex surface. A donut-shaped stainless steel ring placed on tension by springs braces the peripheral tissue. A circular corneal incision is created of a desired thickness by a suction trephine, and a crescent blade is utilized to peripherally dissect a donut-shaped keratolimbal allograft. This device facilitated the harvesting of the keratolimbal allograft tissue from four eye bank donor practice corneoscleral buttons and was then used to successfully procure grafts from six corneoscleral buttons used in three keratolimbal allograft procedures in three patients, one each with aniridia, alkali burn, and drug-induced limbal stem cell deficiency. The described device effectively facilitates procurement of corneoscleral buttons for keratolimbal allograft procedures. It appears to offer advantages over freehanded techniques and previously described devices used for the same purpose.

Original languageEnglish
Pages (from-to)212-214
Number of pages3
JournalAmerican Journal of Ophthalmology
Volume139
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Eye Banks
Allografts
Stem Cells
Tissue Donors
Equipment and Supplies
Suction
Aniridia
Transplants
Equipment Design
Braces
Eye Diseases
Stainless Steel
Alkalies
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Ophthalmology

Cite this

A device to facilitate limbal stem cell procurement from eye bank donor tissue for keratolimbal allograft procedures. / Meisler, David M.; Perez Quinones, Victor L; Proudfit, James.

In: American Journal of Ophthalmology, Vol. 139, No. 1, 01.01.2005, p. 212-214.

Research output: Contribution to journalArticle

Meisler, David M. ; Perez Quinones, Victor L ; Proudfit, James. / A device to facilitate limbal stem cell procurement from eye bank donor tissue for keratolimbal allograft procedures. In: American Journal of Ophthalmology. 2005 ; Vol. 139, No. 1. pp. 212-214.
@article{9c1fbc2f3eba49c9a1b50a8d6a012fe6,
title = "A device to facilitate limbal stem cell procurement from eye bank donor tissue for keratolimbal allograft procedures",
abstract = "To develop a device that facilitates the procurement of corneal limbal stem cell grafts for keratolimbal allograft procedures used in the treatment of ocular surface disease associated with stem cell deficiency. Description of device design and technique for use. The device is composed of a pedestal with a convex surface mounted to a flat platform. A corneoscleral button placed endothelial side down and centrally upon the convexity is secured by suction conveyed through a hollowed core in the pedestal that connects to fenestrated openings on the convex surface. A donut-shaped stainless steel ring placed on tension by springs braces the peripheral tissue. A circular corneal incision is created of a desired thickness by a suction trephine, and a crescent blade is utilized to peripherally dissect a donut-shaped keratolimbal allograft. This device facilitated the harvesting of the keratolimbal allograft tissue from four eye bank donor practice corneoscleral buttons and was then used to successfully procure grafts from six corneoscleral buttons used in three keratolimbal allograft procedures in three patients, one each with aniridia, alkali burn, and drug-induced limbal stem cell deficiency. The described device effectively facilitates procurement of corneoscleral buttons for keratolimbal allograft procedures. It appears to offer advantages over freehanded techniques and previously described devices used for the same purpose.",
author = "Meisler, {David M.} and {Perez Quinones}, {Victor L} and James Proudfit",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.ajo.2004.07.037",
language = "English",
volume = "139",
pages = "212--214",
journal = "American Journal of Ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
number = "1",

}

TY - JOUR

T1 - A device to facilitate limbal stem cell procurement from eye bank donor tissue for keratolimbal allograft procedures

AU - Meisler, David M.

AU - Perez Quinones, Victor L

AU - Proudfit, James

PY - 2005/1/1

Y1 - 2005/1/1

N2 - To develop a device that facilitates the procurement of corneal limbal stem cell grafts for keratolimbal allograft procedures used in the treatment of ocular surface disease associated with stem cell deficiency. Description of device design and technique for use. The device is composed of a pedestal with a convex surface mounted to a flat platform. A corneoscleral button placed endothelial side down and centrally upon the convexity is secured by suction conveyed through a hollowed core in the pedestal that connects to fenestrated openings on the convex surface. A donut-shaped stainless steel ring placed on tension by springs braces the peripheral tissue. A circular corneal incision is created of a desired thickness by a suction trephine, and a crescent blade is utilized to peripherally dissect a donut-shaped keratolimbal allograft. This device facilitated the harvesting of the keratolimbal allograft tissue from four eye bank donor practice corneoscleral buttons and was then used to successfully procure grafts from six corneoscleral buttons used in three keratolimbal allograft procedures in three patients, one each with aniridia, alkali burn, and drug-induced limbal stem cell deficiency. The described device effectively facilitates procurement of corneoscleral buttons for keratolimbal allograft procedures. It appears to offer advantages over freehanded techniques and previously described devices used for the same purpose.

AB - To develop a device that facilitates the procurement of corneal limbal stem cell grafts for keratolimbal allograft procedures used in the treatment of ocular surface disease associated with stem cell deficiency. Description of device design and technique for use. The device is composed of a pedestal with a convex surface mounted to a flat platform. A corneoscleral button placed endothelial side down and centrally upon the convexity is secured by suction conveyed through a hollowed core in the pedestal that connects to fenestrated openings on the convex surface. A donut-shaped stainless steel ring placed on tension by springs braces the peripheral tissue. A circular corneal incision is created of a desired thickness by a suction trephine, and a crescent blade is utilized to peripherally dissect a donut-shaped keratolimbal allograft. This device facilitated the harvesting of the keratolimbal allograft tissue from four eye bank donor practice corneoscleral buttons and was then used to successfully procure grafts from six corneoscleral buttons used in three keratolimbal allograft procedures in three patients, one each with aniridia, alkali burn, and drug-induced limbal stem cell deficiency. The described device effectively facilitates procurement of corneoscleral buttons for keratolimbal allograft procedures. It appears to offer advantages over freehanded techniques and previously described devices used for the same purpose.

UR - http://www.scopus.com/inward/record.url?scp=11844292835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11844292835&partnerID=8YFLogxK

U2 - 10.1016/j.ajo.2004.07.037

DO - 10.1016/j.ajo.2004.07.037

M3 - Article

C2 - 15652862

AN - SCOPUS:11844292835

VL - 139

SP - 212

EP - 214

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

SN - 0002-9394

IS - 1

ER -