A culture model to study neuron-Schwann cell-astrocyte interactions

Susana R. Cerqueira, Yee Shuan Lee, Mary B Bunge

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

In vitro models using Schwann cell and astrocyte co-cultures have been used to understand the mechanisms underlying the formation of boundaries between these cells in vivo. Schwann cell/astrocyte co-cultures also mimic the in vivo scenario of a transplant in a spinal cord injury site, thereby allowing testing of therapeutic approaches. In this chapter, we describe a triple cell culture system with Schwann cells, astrocytes, and neurons that replicates axon growth from a Schwann cell graft into an astrocyte-rich region. In vitro studies using this model can accelerate the discovery of more effective therapeutic combinations to be used along with Schwann cell transplantation after spinal cord injuries.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages269-279
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Keywords

  • Astrocytes
  • Axon growth
  • Co-cultures
  • Glial barrier
  • Neurons
  • Schwann cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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