A critical role for Xdazl a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus

Douglas W. Houston, Mary Lou L King

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

Xdazl is an RNA component of Xenopus germ plasm and encodes an RNA-binding protein that can act as a functional homologue of Drosophila boule. boule is required for entry into meiotic cell division during fly spermatogenesis. Both Xdazl and boule are related to the human DAZ and DAZL, and murine Dazl genes, which are also involved in gamete differentiation. As suggested from its germ plasm localization, we show here that Xdazl is critically involved in PGC development in Xenopus. Xdazl protein is expressed in the cytoplasm, specifically in the germ plasm, from blastula to early tailbud stages. Specific depletion of maternal Xdazl RNA results in tadpoles lacking, or severely deficient in, primordial germ cells (PGCs). In the absence of Xdazl, PGCs do not successfully migrate from the ventral to the dorsal endoderm and do not reach the dorsal mesentery. Germ plasm aggregation and intracellular movements are normal indicating that the defect occurs after PGC formation. We propose that Xdazl is required for early PGC differentiation and is indirectly necessary for the migration of PGCs through the endoderm. As an RNA-binding protein, Xdazl may regulate translation or expression of factors that mediate migration of PGCs.

Original languageEnglish
Pages (from-to)447-456
Number of pages10
JournalDevelopment
Volume127
Issue number3
StatePublished - Feb 1 2000

Fingerprint

Xenopus
Germ Cells
RNA
Endoderm
RNA-Binding Proteins
Blastula
Mesentery
Spermatogenesis
Diptera
Cell Division
Drosophila
Larva
Cell Differentiation
Cytoplasm
Mothers
Genes

Keywords

  • Germ plasm
  • Localized RNA
  • Primordial germ cell
  • RNA-binding protein
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

A critical role for Xdazl a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus. / Houston, Douglas W.; King, Mary Lou L.

In: Development, Vol. 127, No. 3, 01.02.2000, p. 447-456.

Research output: Contribution to journalArticle

Houston, Douglas W. ; King, Mary Lou L. / A critical role for Xdazl a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus. In: Development. 2000 ; Vol. 127, No. 3. pp. 447-456.
@article{1db5d175f1d740c7a0900fecf88def1a,
title = "A critical role for Xdazl a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus",
abstract = "Xdazl is an RNA component of Xenopus germ plasm and encodes an RNA-binding protein that can act as a functional homologue of Drosophila boule. boule is required for entry into meiotic cell division during fly spermatogenesis. Both Xdazl and boule are related to the human DAZ and DAZL, and murine Dazl genes, which are also involved in gamete differentiation. As suggested from its germ plasm localization, we show here that Xdazl is critically involved in PGC development in Xenopus. Xdazl protein is expressed in the cytoplasm, specifically in the germ plasm, from blastula to early tailbud stages. Specific depletion of maternal Xdazl RNA results in tadpoles lacking, or severely deficient in, primordial germ cells (PGCs). In the absence of Xdazl, PGCs do not successfully migrate from the ventral to the dorsal endoderm and do not reach the dorsal mesentery. Germ plasm aggregation and intracellular movements are normal indicating that the defect occurs after PGC formation. We propose that Xdazl is required for early PGC differentiation and is indirectly necessary for the migration of PGCs through the endoderm. As an RNA-binding protein, Xdazl may regulate translation or expression of factors that mediate migration of PGCs.",
keywords = "Germ plasm, Localized RNA, Primordial germ cell, RNA-binding protein, Xenopus",
author = "Houston, {Douglas W.} and King, {Mary Lou L}",
year = "2000",
month = "2",
day = "1",
language = "English",
volume = "127",
pages = "447--456",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "3",

}

TY - JOUR

T1 - A critical role for Xdazl a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus

AU - Houston, Douglas W.

AU - King, Mary Lou L

PY - 2000/2/1

Y1 - 2000/2/1

N2 - Xdazl is an RNA component of Xenopus germ plasm and encodes an RNA-binding protein that can act as a functional homologue of Drosophila boule. boule is required for entry into meiotic cell division during fly spermatogenesis. Both Xdazl and boule are related to the human DAZ and DAZL, and murine Dazl genes, which are also involved in gamete differentiation. As suggested from its germ plasm localization, we show here that Xdazl is critically involved in PGC development in Xenopus. Xdazl protein is expressed in the cytoplasm, specifically in the germ plasm, from blastula to early tailbud stages. Specific depletion of maternal Xdazl RNA results in tadpoles lacking, or severely deficient in, primordial germ cells (PGCs). In the absence of Xdazl, PGCs do not successfully migrate from the ventral to the dorsal endoderm and do not reach the dorsal mesentery. Germ plasm aggregation and intracellular movements are normal indicating that the defect occurs after PGC formation. We propose that Xdazl is required for early PGC differentiation and is indirectly necessary for the migration of PGCs through the endoderm. As an RNA-binding protein, Xdazl may regulate translation or expression of factors that mediate migration of PGCs.

AB - Xdazl is an RNA component of Xenopus germ plasm and encodes an RNA-binding protein that can act as a functional homologue of Drosophila boule. boule is required for entry into meiotic cell division during fly spermatogenesis. Both Xdazl and boule are related to the human DAZ and DAZL, and murine Dazl genes, which are also involved in gamete differentiation. As suggested from its germ plasm localization, we show here that Xdazl is critically involved in PGC development in Xenopus. Xdazl protein is expressed in the cytoplasm, specifically in the germ plasm, from blastula to early tailbud stages. Specific depletion of maternal Xdazl RNA results in tadpoles lacking, or severely deficient in, primordial germ cells (PGCs). In the absence of Xdazl, PGCs do not successfully migrate from the ventral to the dorsal endoderm and do not reach the dorsal mesentery. Germ plasm aggregation and intracellular movements are normal indicating that the defect occurs after PGC formation. We propose that Xdazl is required for early PGC differentiation and is indirectly necessary for the migration of PGCs through the endoderm. As an RNA-binding protein, Xdazl may regulate translation or expression of factors that mediate migration of PGCs.

KW - Germ plasm

KW - Localized RNA

KW - Primordial germ cell

KW - RNA-binding protein

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0033981002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033981002&partnerID=8YFLogxK

M3 - Article

C2 - 10631166

AN - SCOPUS:0033981002

VL - 127

SP - 447

EP - 456

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 3

ER -