A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc

Zhao Jun Liu, Takahiro Ueda, Tadaaki Miyazaki, Nobuyuki Tanaka, Shinichiro Mine, Yoshiya Tanaka, Tadatsugu Taniguchi, Hirohei Yamamura, Yasuhiro Minami

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33 Scopus citations

Abstract

Cyclin C, a putative G1 cyclin, was originally isolated through its ability to complement a Saccharomyces cerevisiae strain lacking the G1 cyclin gene CLN1-3. Unlike cyclins D1 and E, the other two G1 cyclins obtained by the same approach and subsequently shown to play important roles during the G1/S transition, there is thus far no evidence to support the hypothesis that cyclin C is indeed critical for the promotion of cell cycle progression. In BAF-B03 cells, an interleukin 3 (IL-3)-dependent murine pro- B-cell line, cyclin C gene mRNA was induced at the G1/S phase upon IL-3 stimulation and reached a maximal level in the S phase. Enforced expression of exogenous cyclin C in this cell line failed to alter its growth properties. In the present study, we examined whether cyclin C is capable of cooperating with the cytokine-responsive immediate-early gene products c-Myc and c-Fos in the promotion of cell proliferation. We found that cyclin C is able to cooperate functionally with c-Myc, but not c-Fos, to induce both BAF- B03 cell proliferation in a cytokine-independent fashion and the formation of cell clusters. Furthermore, cyclin C was primarily responsible for the induction of cdc2 gene expression. Our data define a novel role for cyclin C in the regulation of both the G1/S and G2/M phases of the cell cycle, and this effect appears to be independent of the activity of CDK8 in the control of transcription.

Original languageEnglish (US)
Pages (from-to)3445-3454
Number of pages10
JournalMolecular and cellular biology
Volume18
Issue number6
DOIs
StatePublished - Jun 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Liu, Z. J., Ueda, T., Miyazaki, T., Tanaka, N., Mine, S., Tanaka, Y., Taniguchi, T., Yamamura, H., & Minami, Y. (1998). A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc. Molecular and cellular biology, 18(6), 3445-3454. https://doi.org/10.1128/MCB.18.6.3445