Previously published mtDNA cytochrome b sequences for Xyrichthys novacula (Linnaeus) and two morphologically distinct types of Xyrichthys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to identify the two types of Xyrichthys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X. martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichthys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichthys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Aquatic Science