A coordinated approach to cutaneous wound healing: Vibrational microscopy and molecular biology

K. L.Andrew Chan, Guojin Zhang, Marjana Tomic-Canic, Olivera Stojadinovic, Brian Lee, Carol R. Flach, Richard Mendelsohn

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


The repair of cutaneous wounds in the adult body involves a complex series of spatially and temporally organized processes to prevent infection and restore homeostasis. Three characteristic phases of wound repair (inflammation, proliferation including re-epithelialization and remodelling) overlap in time and space. We have utilized a human skin wound-healing model to correlate changes in genotype and pheno-type with infrared (IR) and confocal Raman spectroscopic images during the re-epithelialization of excisional wounds. The experimental protocols validated as IR images clearly delineate the keratin-rich migrating epithelial tongue from the collagen-rich wound bed. Multivariate statistical analysis of IR datasets acquired 6 days post-wounding reveal subtle spectral differences that map to distinct spatial distributions, which are correlated with immunofluorescent staining patterns of different keratin types. Images computed within collagen-rich regions expose complementary spatial patterns and identify elastin in the wound bed. The temporal sequence of events is explored through a comparison of gene array analysis with confocal Raman microscopy. Our approach demonstrates the feasibility of acquiring detailed molecular structure information from the various proteins and their subclasses involved in the wound-healing process.

Original languageEnglish (US)
Pages (from-to)2145-2154
Number of pages10
JournalJournal of Cellular and Molecular Medicine
Issue number5B
StatePublished - Oct 2008
Externally publishedYes


  • Confocal Raman microscopy
  • Infrared imaging
  • Migrating epithelial tongue
  • Wound healing

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine


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