Abstract
A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
Original language | English (US) |
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Pages (from-to) | 229-235 |
Number of pages | 7 |
Journal | Biochemical Engineering Journal |
Volume | 19 |
Issue number | 3 |
DOIs | |
State | Published - Jul 29 2004 |
Externally published | Yes |
Keywords
- Bioreactors
- Carbon dioxide fixation start-up
- Immobilized enzymes
- RuBP production
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Chemical Engineering(all)