A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

Sumana Bhattacharya, Amiya Nayak, James Gomes, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalBiochemical Engineering Journal
Volume19
Issue number3
DOIs
StatePublished - Jul 29 2004
Externally publishedYes

Fingerprint

Glucose
Phosphates
Phosphogluconate Dehydrogenase
Hexokinase
Glucosephosphate Dehydrogenase
Kinetic parameters
Enzymes
ribulose-1,5 diphosphate
Oxidoreductases
ribosephosphate isomerase
phosphoribulokinase

Keywords

  • Bioreactors
  • Carbon dioxide fixation start-up
  • Immobilized enzymes
  • RuBP production

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Chemical Engineering(all)

Cite this

A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose. / Bhattacharya, Sumana; Nayak, Amiya; Gomes, James; Bhattacharya, Sanjoy K.

In: Biochemical Engineering Journal, Vol. 19, No. 3, 29.07.2004, p. 229-235.

Research output: Contribution to journalArticle

Bhattacharya, Sumana ; Nayak, Amiya ; Gomes, James ; Bhattacharya, Sanjoy K. / A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose. In: Biochemical Engineering Journal. 2004 ; Vol. 19, No. 3. pp. 229-235.
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