A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

Sumana Bhattacharya; Amiya Nayak; James Gomes; Sanjoy K. Bhattacharya

doi:10.1016/j.bej.2004.02.002

A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

Sumana Bhattacharya, Amiya Nayak, James Gomes, Sanjoy K. Bhattacharya

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.

Original language	English (US)
Pages (from-to)	229-235
Number of pages	7
Journal	Biochemical Engineering Journal
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2004.02.002
State	Published - Jul 29 2004
Externally published	Yes

Keywords

Bioreactors
Carbon dioxide fixation start-up
Immobilized enzymes
RuBP production

ASJC Scopus subject areas

Biotechnology
Bioengineering
Chemical Engineering(all)

Access to Document

10.1016/j.bej.2004.02.002

Fingerprint Dive into the research topics of 'A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose'. Together they form a unique fingerprint.

Cite this

Bhattacharya, S., Nayak, A., Gomes, J., & Bhattacharya, S. K. (2004). A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose. Biochemical Engineering Journal, 19(3), 229-235. https://doi.org/10.1016/j.bej.2004.02.002

@article{5aa91b7ed8fc41dfbd8437c2fe9aae9a,

title = "A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose",

abstract = "A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.",

keywords = "Bioreactors, Carbon dioxide fixation start-up, Immobilized enzymes, RuBP production",

author = "Sumana Bhattacharya and Amiya Nayak and James Gomes and Bhattacharya, {Sanjoy K.}",

year = "2004",

month = jul,

day = "29",

doi = "10.1016/j.bej.2004.02.002",

language = "English (US)",

volume = "19",

pages = "229--235",

journal = "Biochemical Engineering Journal",

issn = "1369-703X",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

AU - Bhattacharya, Sumana

AU - Nayak, Amiya

AU - Gomes, James

AU - Bhattacharya, Sanjoy K.

PY - 2004/7/29

Y1 - 2004/7/29

N2 - A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.

AB - A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.

KW - Bioreactors

KW - Carbon dioxide fixation start-up

KW - Immobilized enzymes

KW - RuBP production

UR - http://www.scopus.com/inward/record.url?scp=2542434170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542434170&partnerID=8YFLogxK

U2 - 10.1016/j.bej.2004.02.002

DO - 10.1016/j.bej.2004.02.002

M3 - Article

AN - SCOPUS:2542434170

VL - 19

SP - 229

EP - 235

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

IS - 3

ER -