A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

Sumana Bhattacharya; Amiya Nayak; James Gomes; Sanjoy K. Bhattacharya

doi:10.1016/j.bej.2004.02.002

A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose

Sumana Bhattacharya, Amiya Nayak, James Gomes, Sanjoy K. Bhattacharya

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.

Original language	English (US)
Pages (from-to)	229-235
Number of pages	7
Journal	Biochemical Engineering Journal
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2004.02.002
State	Published - Jul 29 2004
Externally published	Yes

Keywords

Bioreactors
Carbon dioxide fixation start-up
Immobilized enzymes
RuBP production

ASJC Scopus subject areas

Biotechnology
Bioengineering
Chemical Engineering(all)

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10.1016/j.bej.2004.02.002

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Cite this

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title = "A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose",

abstract = "A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.",

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AU - Bhattacharya, Sumana

AU - Nayak, Amiya

AU - Gomes, James

AU - Bhattacharya, Sanjoy K.

PY - 2004/7/29

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