Abstract
A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
| Original language | English |
|---|---|
| Pages (from-to) | 229-235 |
| Number of pages | 7 |
| Journal | Biochemical Engineering Journal |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 29 2004 |
| Externally published | Yes |
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Keywords
- Bioreactors
- Carbon dioxide fixation start-up
- Immobilized enzymes
- RuBP production
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Chemical Engineering(all)
Cite this
A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose. / Bhattacharya, Sumana; Nayak, Amiya; Gomes, James; Bhattacharya, Sanjoy K.
In: Biochemical Engineering Journal, Vol. 19, No. 3, 29.07.2004, p. 229-235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A continuous process for production of D-ribulose-1,5-bisphosphate from D-glucose
AU - Bhattacharya, Sumana
AU - Nayak, Amiya
AU - Gomes, James
AU - Bhattacharya, Sanjoy K
PY - 2004/7/29
Y1 - 2004/7/29
N2 - A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
AB - A continuous bioprocess for production of D-ribulose-1,5-bisphosphate (RuBP) using D-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from D-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
KW - Bioreactors
KW - Carbon dioxide fixation start-up
KW - Immobilized enzymes
KW - RuBP production
UR - http://www.scopus.com/inward/record.url?scp=2542434170&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542434170&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2004.02.002
DO - 10.1016/j.bej.2004.02.002
M3 - Article
AN - SCOPUS:2542434170
VL - 19
SP - 229
EP - 235
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 3
ER -