A conserved downstream element defines a new class of RNA polymerase II promoters

Tan Ince, Kathleen W. Scotto

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P- glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.

Original languageEnglish
Pages (from-to)30249-30252
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number51
DOIs
StatePublished - Dec 22 1995
Externally publishedYes

Fingerprint

RNA Polymerase II
P-Glycoprotein
Transcription
Transcription Initiation Site
Protein Binding
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

A conserved downstream element defines a new class of RNA polymerase II promoters. / Ince, Tan; Scotto, Kathleen W.

In: Journal of Biological Chemistry, Vol. 270, No. 51, 22.12.1995, p. 30249-30252.

Research output: Contribution to journalArticle

@article{085704ed1e6246a39cd462639c910173,
title = "A conserved downstream element defines a new class of RNA polymerase II promoters",
abstract = "Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P- glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.",
author = "Tan Ince and Scotto, {Kathleen W.}",
year = "1995",
month = "12",
day = "22",
doi = "10.1074/jbc.270.51.30249",
language = "English",
volume = "270",
pages = "30249--30252",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "51",

}

TY - JOUR

T1 - A conserved downstream element defines a new class of RNA polymerase II promoters

AU - Ince, Tan

AU - Scotto, Kathleen W.

PY - 1995/12/22

Y1 - 1995/12/22

N2 - Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P- glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.

AB - Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P- glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.

UR - http://www.scopus.com/inward/record.url?scp=0029586499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029586499&partnerID=8YFLogxK

U2 - 10.1074/jbc.270.51.30249

DO - 10.1074/jbc.270.51.30249

M3 - Article

C2 - 8530439

AN - SCOPUS:0029586499

VL - 270

SP - 30249

EP - 30252

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 51

ER -