A conjugate of doxorubicin and an analog of Luteinizing Hormone-Releasing Hormone shows increased efficacy against oral and laryngeal cancers

Linda J. Krebs, Xiaopeng Wang, Attila Nagy, Andrew V. Schally, Paras N. Prasad, Charles Liebow

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A doxorubicin and [D-Lys(6)]Luteinizing Hormone-Releasing Hormone (LH-RH) conjugate, AN-152, was designed to target LH-RH receptor positive cells. AN-152 is more potent against a specific group of cancers than doxorubicin and has less peripheral toxicity. This therapy is potentially efficacious against many other types of malignancies. Here, AN-152 was tested on oral (KB) and laryngeal (HEp-2) carcinoma cells. LH-RH receptor presence was demonstrated by displacement binding assays. Cells were treated with 10 nM EGF or left untreated, then exposed to 0-10 microM AN-152. Cytotoxicity was assessed by MTT assay to determine ED(50) values. AN-152 association with the cells was monitored using two-photon laser scanning microscopy. AN-152 was more potent than doxorubicin in both KB and HEp-2 cell lines (P<0.05). Up-regulation of LH-RH receptors by epidermal growth factor (EGF) increased the entry and potency of AN-152 in KB cells, but not in HEp-2 cells. This novel approach may be effective against a variety of cancers.

Original languageEnglish (US)
Pages (from-to)657-663
Number of pages7
JournalOral oncology
Volume38
Issue number7
DOIs
StatePublished - Oct 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

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