Abstract
The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.
| Original language | English |
|---|---|
| Pages (from-to) | 160-170 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 193 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
A conditional mutant of vRel containing sequences from the human estrogen receptor. / Capobianco, Anthony J; Gilmore, T. D.
In: Virology, Vol. 193, No. 1, 01.01.1993, p. 160-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A conditional mutant of vRel containing sequences from the human estrogen receptor
AU - Capobianco, Anthony J
AU - Gilmore, T. D.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.
AB - The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.
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U2 - 10.1006/viro.1993.1112
DO - 10.1006/viro.1993.1112
M3 - Article
VL - 193
SP - 160
EP - 170
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -