A conditional mutant of vRel containing sequences from the human estrogen receptor

Anthony J Capobianco, T. D. Gilmore

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.

Original languageEnglish
Pages (from-to)160-170
Number of pages11
JournalVirology
Volume193
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Estrogen Receptors
Estrogens
Chickens
Embryonic Structures
Fibroblasts
rel Genes
Spleen
Electrophoretic Mobility Shift Assay
Retroviridae
Cytoplasm
Maintenance
Ligands
Cell Line
DNA
Growth
Genes
estrophilin

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

A conditional mutant of vRel containing sequences from the human estrogen receptor. / Capobianco, Anthony J; Gilmore, T. D.

In: Virology, Vol. 193, No. 1, 01.01.1993, p. 160-170.

Research output: Contribution to journalArticle

@article{47cb54d4292246bbaf68543c17b56fd6,
title = "A conditional mutant of vRel containing sequences from the human estrogen receptor",
abstract = "The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.",
author = "Capobianco, {Anthony J} and Gilmore, {T. D.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1006/viro.1993.1112",
language = "English",
volume = "193",
pages = "160--170",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A conditional mutant of vRel containing sequences from the human estrogen receptor

AU - Capobianco, Anthony J

AU - Gilmore, T. D.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.

AB - The mechanism by which the v-rel oncogene of the avian Rev-T retrovirus transforms chicken spleen cells is not known. We have created v-rel mutants that show conditional properties by fusing sequences encoding the ligand-binding domain of the human estrogen receptor (ER) in-frame at the 3' end of the v-rel oncogene. Two vRel-ER fusion proteins showed estrogen-dependent subcellular localization in chicken embryo fibroblasts (CEF): vRel-ER proteins were located in the cytoplasm of CEF in the absence of estrogen and were located in the nucleus of CEF in the presence of estrogen. Wild-type vRel was located in the nucleus of CEF in the presence or absence of estrogen. Mobility shift assays using extracts from infected CEF showed that the ability of vRel-ER to bind DNA was also dependent on estrogen. However, the ability of vRel-ER to repress transcription from κB site-containing promoters was not dependent on estrogen. Finally, we were able to isolate a vRel-ER-transformed avian spleen cell line whose growth is dependent on estrogen; this indicates that a vRel function is needed for both the initiation and the maintenance of the transformed state. The vRel-ER protein may be useful for determining genes controlled by vRel.

UR - http://www.scopus.com/inward/record.url?scp=0027292798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027292798&partnerID=8YFLogxK

U2 - 10.1006/viro.1993.1112

DO - 10.1006/viro.1993.1112

M3 - Article

VL - 193

SP - 160

EP - 170

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -