A comprehensive Plasmodium falciparum protein interaction map reveals a distinct architecture of a core interactome

Stefan Wuchty, John H. Adams, Michael T. Ferdig

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We derive a map of protein interactions in the parasite Plasmodium falciparum from conserved interactions in Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, and Escherichia coli and pool them with experimental interaction data. The application of a cliquepercolation algorithm allows us to find overlapping clusters, strongly correlated with yeast specific conserved protein complexes. Such clusters contain core activities that govern gene expression, largely dominated by components of protein production and degradation processes as well as RNA metabolism. A critical role of protein hubs in the interactome of P. falciparum is supported by their appearance in multiple clusters and the tendencies of their interactions to reach into many distinct protein clusters. Parasite proteins with a human ortholog tend to appear in single complexes. Annotating each protein with the stage where it is maximally expressed we observe a high level of cluster integrity in the ring stage. While we find no signal in the trophozoite phase, expression patterns are reversed in the schizont phase, implying a preponderance of parasite specific functions in this late, invasive schizont stage. As such, the inference of potential protein interactions and their analysis contributes to our understanding of the parasite, indicating basic pathways and processes as unique targets for therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)1841-1849
Number of pages9
JournalProteomics
Volume9
Issue number7
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

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Keywords

  • Interactome
  • Malaria
  • Networks
  • Parasite
  • Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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