TY - JOUR
T1 - A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis
AU - Paus, Ralf
AU - Müller-Röver, Sven
AU - Van Der Veen, Carina
AU - Maurer, Marcus
AU - Eichmüller, Stefan
AU - Ling, Gao
AU - Hofmann, Udo
AU - Foitzik, Kerstin
AU - Mecklenburg, Lars
AU - Handjiski, Bori
N1 - Funding Information:
The excellent technical assistance of R. Pliet and E. Hagen, and the most helpful critical suggestions of J.P. Sundberg are gratefully acknowledged. This study was supported in parts by grants from Deutsche Forschungsgemeinschaft (Pa 345/8–2) and the E.U. (Brite Euram Project No. BE 97–4301) to R.P.
PY - 1999
Y1 - 1999
N2 - Numerous spontaneous and experimentally induced mouse mutations develop a hair phenotype, which is often associated with more or less discrete abnormalities in hair follicle development. In order to recognize these, it is critically important to be able to determine and to classify accurately the major stages of normal murine hair follicle morphogenesis. As an aid, we propose a pragmatic and comprehensive guide, modified after previous suggestions by Hardy, and provide a list of easily recognizable classification criteria, illustrated by representative micrographs. Basic and more advanced criteria are distinguished, the former being applicable to all mouse strains and requiring only simple histologic stains (hematoxylin and eosin, Giemsa, periodic acid Schiff, alkaline phosphatase activity), the latter serving as auxiliary criteria, which require a pigmented mouse strain (like C57BL/6J) or immunohistochemistry (interleukin-1 receptor type I, transforming growth factor-β receptor type II). In addition, we present simplified, computer-generated schematic drawings for the standardized recording and reporting of gene and antigen expression patterns during hair follicle development. This classification aid serves as a basic introduction into the field of hair follicle morphogenesis, aims at standardizing the presentation of related hair research data, and should become a useful tool when screening new mouse mutants for discrete abnormalities of hair follicle morphogenesis (compared with the respective wild type) in a highly reproducible, easily applicable, and quantifiable manner.
AB - Numerous spontaneous and experimentally induced mouse mutations develop a hair phenotype, which is often associated with more or less discrete abnormalities in hair follicle development. In order to recognize these, it is critically important to be able to determine and to classify accurately the major stages of normal murine hair follicle morphogenesis. As an aid, we propose a pragmatic and comprehensive guide, modified after previous suggestions by Hardy, and provide a list of easily recognizable classification criteria, illustrated by representative micrographs. Basic and more advanced criteria are distinguished, the former being applicable to all mouse strains and requiring only simple histologic stains (hematoxylin and eosin, Giemsa, periodic acid Schiff, alkaline phosphatase activity), the latter serving as auxiliary criteria, which require a pigmented mouse strain (like C57BL/6J) or immunohistochemistry (interleukin-1 receptor type I, transforming growth factor-β receptor type II). In addition, we present simplified, computer-generated schematic drawings for the standardized recording and reporting of gene and antigen expression patterns during hair follicle development. This classification aid serves as a basic introduction into the field of hair follicle morphogenesis, aims at standardizing the presentation of related hair research data, and should become a useful tool when screening new mouse mutants for discrete abnormalities of hair follicle morphogenesis (compared with the respective wild type) in a highly reproducible, easily applicable, and quantifiable manner.
KW - 6J mouse
KW - Alkaline phosphatase
KW - C57BL
KW - Dermal papilla
KW - Interleukin-1 receptor
KW - Transforming growth factor-β receptor
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U2 - 10.1046/j.1523-1747.1999.00740.x
DO - 10.1046/j.1523-1747.1999.00740.x
M3 - Review article
C2 - 10504436
AN - SCOPUS:0344054050
VL - 113
SP - 523
EP - 532
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 4
ER -