A comparison of two microbial detection methods used in aseptic processing of musculoskeletal allograft tissues

Jeremy A. Dennis, Octavio V. Martinez, David C. Landy, Theodore I. Malinin, Paul R. Morris, William P. Fox, Billy E. Buck, H. Thomas Temple

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Tissues from 78 musculoskeletal donors were concurrently tested for microorganisms using both a swab and liquid culture method. An aggregate total of 20 organisms were detected by both methods. The swab detected 4/20 organisms while the liquid culture detected 18/20 organisms. The swab method yielded sensitivity and negative predictive values of 20 and 92.3%, respectively. Comparatively, the liquid culture displayed a sensitivity of 90% and a negative predictive value of 99%. These results clearly demonstrate that the liquid culture method is superior to swab cultures in microbial detection. Additional studies are necessary to determine the optimal culture conditions for different types of tissues when utilizing the liquid culture method.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalCell and Tissue Banking
Volume12
Issue number1
DOIs
StatePublished - Feb 2011

Keywords

  • Allograft
  • Culture
  • Microbial
  • Musculoskeletal tissue
  • Sensitivity

ASJC Scopus subject areas

  • Transplantation
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

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