A comparison of immunohistochemistry, two-color immunofluorescence, and flow cytometry with cell sorting for the detection of micrometastatic breast cancer in the bone marrow

J. J. Vredenburgh, O. Silva, C. Tyer, K. DeSombre, A. Abou-Ghalia, M. Cook, L. Layfield, W. P. Peters, R. C. Bast

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A significant percentage of women with primary breast cancer have micrometastatic disease in the bone marrow. Bone marrow involvement may be an adverse prognostic factor, and more aggressive therapy may be indicated for these patients. There are a number of different techniques and antibodies used to detect tumor cells in the bone marrow. We used the same panels of four antibreast cancer antibodies and compared three immunodetection techniques: two-color immunofluorescence, immunohistochemical staining, and fluorescence-activated cell sorting with cytologic examination of the sorted cells. The two-color immunofluorescence technique was superior and consistently detected one tumor cell contaminating one million normal bone marrow cells and had no reactivity with normal bone marrow.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalJournal of Hematotherapy and Stem Cell Research
Volume5
Issue number1
StatePublished - Feb 1 1996

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Bone Neoplasms
Fluorescent Antibody Technique
Flow Cytometry
Color
Bone Marrow
Immunohistochemistry
Breast Neoplasms
Bone Marrow Cells
Bone Marrow Diseases
Neoplasms
Antibodies
Staining and Labeling
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Hematology

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A comparison of immunohistochemistry, two-color immunofluorescence, and flow cytometry with cell sorting for the detection of micrometastatic breast cancer in the bone marrow. / Vredenburgh, J. J.; Silva, O.; Tyer, C.; DeSombre, K.; Abou-Ghalia, A.; Cook, M.; Layfield, L.; Peters, W. P.; Bast, R. C.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 5, No. 1, 01.02.1996, p. 57-62.

Research output: Contribution to journalArticle

Vredenburgh, J. J. ; Silva, O. ; Tyer, C. ; DeSombre, K. ; Abou-Ghalia, A. ; Cook, M. ; Layfield, L. ; Peters, W. P. ; Bast, R. C. / A comparison of immunohistochemistry, two-color immunofluorescence, and flow cytometry with cell sorting for the detection of micrometastatic breast cancer in the bone marrow. In: Journal of Hematotherapy and Stem Cell Research. 1996 ; Vol. 5, No. 1. pp. 57-62.
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