A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels

Guang Shing Perng, R. Dino Rulli, David L. Wilson, G. W. Perry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.

Original languageEnglish
Pages (from-to)387-392
Number of pages6
JournalAnalytical Biochemistry
Volume173
Issue number2
DOIs
StatePublished - Jan 1 1988

Fingerprint

X ray films
Dimethyl Sulfoxide
Electrophoresis
Gels
X-Ray Film
Autoradiography
Proteins
2,5-diphenyloxazole
polyacrylamide gels

Keywords

  • autoradiography
  • fluorography
  • gel electrophoresis
  • proteins
  • two-dimensional gels
  • X-ray film

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels. / Perng, Guang Shing; Rulli, R. Dino; Wilson, David L.; Perry, G. W.

In: Analytical Biochemistry, Vol. 173, No. 2, 01.01.1988, p. 387-392.

Research output: Contribution to journalArticle

Perng, Guang Shing ; Rulli, R. Dino ; Wilson, David L. ; Perry, G. W. / A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels. In: Analytical Biochemistry. 1988 ; Vol. 173, No. 2. pp. 387-392.
@article{3657f1b3ed8d470994068d43694e9c39,
title = "A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels",
abstract = "Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.",
keywords = "autoradiography, fluorography, gel electrophoresis, proteins, two-dimensional gels, X-ray film",
author = "Perng, {Guang Shing} and Rulli, {R. Dino} and Wilson, {David L.} and Perry, {G. W.}",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0003-2697(88)90204-7",
language = "English",
volume = "173",
pages = "387--392",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels

AU - Perng, Guang Shing

AU - Rulli, R. Dino

AU - Wilson, David L.

AU - Perry, G. W.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.

AB - Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.

KW - autoradiography

KW - fluorography

KW - gel electrophoresis

KW - proteins

KW - two-dimensional gels

KW - X-ray film

UR - http://www.scopus.com/inward/record.url?scp=0023690220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023690220&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(88)90204-7

DO - 10.1016/0003-2697(88)90204-7

M3 - Article

C2 - 3189817

AN - SCOPUS:0023690220

VL - 173

SP - 387

EP - 392

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -