A comparison of fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels

Guang Shing Perng, R. Dino Rulli, David L. Wilson, G. W. Perry

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Eight different methods of fluorographic enhancement of sensitivity to 35S decay after gel electrophoresis were compared. Using Kodak X-Omat AR X-ray film, we found that some fluors were about equivalent to 2,5-diphenyloxazole/dimethyl sulfoxide embedding, whereas several other fluors were not quite as effective, but still were significantly more sensitive than control autoradiography. The most sensitive procedures can yield a detectable darkening of film with less than 1 dpm/mm2 of 35S after a 1-week exposure.

Original languageEnglish (US)
Pages (from-to)387-392
Number of pages6
JournalAnalytical Biochemistry
Volume173
Issue number2
DOIs
StatePublished - Sep 1988

Keywords

  • autoradiography
  • fluorography
  • gel electrophoresis
  • proteins
  • two-dimensional gels
  • X-ray film

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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