A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles

Na Li, Lu Yu, Jiaqi Zou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Oligonucleotide-functionalizing noble metal nanoparticles (NMNPs) have resulted in many interesting applications in engineering, as well as in medicine. Typically, NMNPs are functionalized via thiol-metal interaction using thiol-labeled oligonucleotides. Previous studies have revealed that strong interactions exist between oligonucleotide bases and noble metal surface and these interactions are sequence-dependent. This has provided an alternative way to functionalize NMNPs with unlabeled oligonucleotides. In order to fully utilize the spontaneous interactions between oligonucleotides and NMNPs, quantitative assessment of the interaction strength between different oligonucleotides and NMNPs will be necessary. Existing methods that have been used to determine the interaction strength usually rely on direct quantification of the amount of oligonucleotides adsorbed on NMNPs, and tend to be labor- and time-consuming. Herein we develop a salt titration based colorimetric method as a simple and high-throughput alternative to the existing methods. First, this new method was demonstrated by applying to determine the dissociation constant between gold nanoparticles and DNA oligonucleotides. Then the new method was used to study the sequence and length dependence of NMNP-oligonucleotide interactions.

Original languageEnglish (US)
Title of host publication9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
PublisherIEEE Computer Society
Pages43-46
Number of pages4
Volume2016-June
ISBN (Electronic)9781467396714
DOIs
StatePublished - Jun 15 2016
Event9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 - Honolulu, United States
Duration: Nov 15 2015Nov 18 2015

Other

Other9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
CountryUnited States
CityHonolulu
Period11/15/1511/18/15

Fingerprint

Metal Nanoparticles
Metal nanoparticles
Oligonucleotides
Precious metals
Adsorption
Sulfhydryl Compounds
Metals
Titration
Gold
Nanoparticles
Medicine
DNA
Salts
Throughput
Personnel

Keywords

  • Adsorption strength
  • Colorimetric detection
  • Critical coagulation concentration
  • Gold nanoparticles
  • Noble metal nanoparticles
  • Oligonucleotides
  • Salt titration
  • Silver nanoparticles

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Li, N., Yu, L., & Zou, J. (2016). A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 (Vol. 2016-June, pp. 43-46). [7492502] IEEE Computer Society. https://doi.org/10.1109/NANOMED.2015.7492502

A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles. / Li, Na; Yu, Lu; Zou, Jiaqi.

9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June IEEE Computer Society, 2016. p. 43-46 7492502.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Li, N, Yu, L & Zou, J 2016, A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles. in 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. vol. 2016-June, 7492502, IEEE Computer Society, pp. 43-46, 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015, Honolulu, United States, 11/15/15. https://doi.org/10.1109/NANOMED.2015.7492502
Li N, Yu L, Zou J. A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June. IEEE Computer Society. 2016. p. 43-46. 7492502 https://doi.org/10.1109/NANOMED.2015.7492502
Li, Na ; Yu, Lu ; Zou, Jiaqi. / A colorimetric method for assessing the adsorption strength of oligonucleotides on noble metal nanoparticles. 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June IEEE Computer Society, 2016. pp. 43-46
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