A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II

M. Aboud, D. W. Golde, N. Bersch, Joseph D Rosenblatt, I. S Y Chen

Research output: Contribution to journalArticle

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Abstract

We report here the development of a rapid and quantitative method for measuring in vitro T cell transformation by human T cell leukemia viruses type I (HTLV-I) and type II (HTLV-II). This method is based on our finding that cocultivation of lethally irradiated HTLV-producing cells with peripheral blood lymphocytes (PBLs) preactivated for 24 hours with phytohemagglutinin and interleukin-2 (IL-2) induces colony formation in methylcellulose-containing medium. Colonies of about 200 cells can be clearly distinguished from background aggregates within four to six days after cocultivation. These colonies gradually increase in size and reach 300 to 1,000 cells within 14 days after cocultivation. Cells of these colonies were infected, as evidenced by expression of viral p19 antigen and the presence of HTLV proviral sequences. These cells proved to be transformed in terms of IL-2-independent continuous growth in liquid medium. Colony formation was found to depend in a linear fashion upon the percentage of the infected cells present in the irradiated cell population and is sufficiently sensitive to detect as few as 1% of virus-producing cells.

Original languageEnglish
Pages (from-to)432-436
Number of pages5
JournalBlood
Volume70
Issue number2
StatePublished - Dec 1 1987
Externally publishedYes

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Human T-lymphotropic virus 2
Human T-lymphotropic virus 1
T-cells
Viruses
Deltaretrovirus Antigens
Interleukin-2
Assays
Methylcellulose
Lymphocytes
Phytohemagglutinins
Blood
Coculture Techniques
Cells
Liquids
In Vitro Techniques
Viral Antigens
T-Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Aboud, M., Golde, D. W., Bersch, N., Rosenblatt, J. D., & Chen, I. S. Y. (1987). A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II. Blood, 70(2), 432-436.

A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II. / Aboud, M.; Golde, D. W.; Bersch, N.; Rosenblatt, Joseph D; Chen, I. S Y.

In: Blood, Vol. 70, No. 2, 01.12.1987, p. 432-436.

Research output: Contribution to journalArticle

Aboud, M, Golde, DW, Bersch, N, Rosenblatt, JD & Chen, ISY 1987, 'A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II', Blood, vol. 70, no. 2, pp. 432-436.
Aboud, M. ; Golde, D. W. ; Bersch, N. ; Rosenblatt, Joseph D ; Chen, I. S Y. / A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II. In: Blood. 1987 ; Vol. 70, No. 2. pp. 432-436.
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