A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration

Eva Maria Engelhardt, Lionel A. Micol, Stephanie Houis, Florian M. Wurm, Jöns Hilborn, Jeffrey A. Hubbell, Peter Frey

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intestinal submucosa, they offer a limited mechanical strength even if crosslinking can enhance their mechanical properties. A more cell-friendly way to increase material strength is to combine synthetic polymer meshes with plastic compressed collagen gels. This work describes the potential of plastic compressed collagen-poly(lactic acid-co-e{open}-caprolactone) (PLAC) hybrids as scaffolds for bladder tissue regeneration. Human bladder smooth muscle and urothelial cells were cultured on and inside collagen-PLAC hybrids in vitro. Scaffolds were analyzed by electron microscopy, histology, immunohistochemistry, and AlamarBlue assay. Both cell types proliferated in and on the hybrid, forming dense cell layers on top after two weeks. Furthermore, hybrids were implanted subcutaneously in the backs of nude mice. Host cell infiltration, scaffold degradation, and the presence of the seeded bladder cells were analyzed. Hybrids showed a lower inflammatory reaction in vivo than PLAC meshes alone, and first signs of polymer degradation were visible at six months. Collagen-PLAC hybrids have potential for bladder tissue regeneration, as they show efficient cell seeding, proliferation, and good mechanical properties.

Original languageEnglish
Pages (from-to)3969-3976
Number of pages8
JournalBiomaterials
Volume32
Issue number16
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Tissue Scaffolds
Tissue regeneration
Lactic acid
Collagen
Scaffolds
Regeneration
Urinary Bladder
Lactic Acid
Scaffolds (biology)
Plastics
Strength of materials
Polymers
Cell Proliferation
Degradation
Mechanical properties
Histology
Infiltration
Nude Mice
Crosslinking
Electron microscopy

Keywords

  • Bladder tissue engineering
  • Collagen
  • Copolymer
  • In vitro test
  • In vivo test
  • Scaffold

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Engelhardt, E. M., Micol, L. A., Houis, S., Wurm, F. M., Hilborn, J., Hubbell, J. A., & Frey, P. (2011). A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration. Biomaterials, 32(16), 3969-3976. https://doi.org/10.1016/j.biomaterials.2011.02.012

A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration. / Engelhardt, Eva Maria; Micol, Lionel A.; Houis, Stephanie; Wurm, Florian M.; Hilborn, Jöns; Hubbell, Jeffrey A.; Frey, Peter.

In: Biomaterials, Vol. 32, No. 16, 01.06.2011, p. 3969-3976.

Research output: Contribution to journalArticle

Engelhardt, EM, Micol, LA, Houis, S, Wurm, FM, Hilborn, J, Hubbell, JA & Frey, P 2011, 'A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration', Biomaterials, vol. 32, no. 16, pp. 3969-3976. https://doi.org/10.1016/j.biomaterials.2011.02.012
Engelhardt, Eva Maria ; Micol, Lionel A. ; Houis, Stephanie ; Wurm, Florian M. ; Hilborn, Jöns ; Hubbell, Jeffrey A. ; Frey, Peter. / A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration. In: Biomaterials. 2011 ; Vol. 32, No. 16. pp. 3969-3976.
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